Abstract: The interaction of bovine serum albumin(BSA) with perfluorooctane sulfonate(PFOS) was investigated with resonance light scattering(RLS) spectroscopy. A RLS method for the determination of PFOS was established. In pH=4.1 Britton-Robinson buffer solution, perfluorooctane sulfonate anions reacted with the protonated BSA by electrostatic forces and hydrophobic interactions to form ion-association complexes and resulted in greatly enhanced resonance light scattering signals characterized by a peak at 285.0 nm. The RLS intensity of system was proportional to the content of PFOS in the range of 0.2~25.0 μmol/L, and the limit of detection was 20.0 nmol/L. The optimum reaction conditions and interference of foreign substances were investigated. This RLS method was applied to the determination of PFOS in environmental samples with RSD≤4.4%.

Key words: bovine serum albumin, perfluorooctane sulfonate, resonance light scattering