Chinese Journal of Applied Chemistry ›› 2016, Vol. 33 ›› Issue (3): 357-363.DOI: 10.11944/j.issn.1000-0518.2016.03.150238

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Synthesis of a Fluorescein Derivative 5-Bromosalicylicaldehyde Fluorescein Hydrazone and the Detection for Copper(Ⅱ) Ion

WU Xinab,MA Juna,NAN Minga,SHUANG Shaomina,DONG Chuana*()   

  1. aInstitute of Environmental Science,School of Chemistry and Chemical Engineering,Shanxi University,Taiyuan 030006,China
    bSchool of Arts and Sciences,Shanxi Agricultural University,Taigu,Shanxi 030801,China
  • Received:2015-07-09 Accepted:2015-11-17 Published:2016-03-02 Online:2016-03-02
  • Contact: DONG Chuan
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21405099, No.21575084), Shanxi Council Scholarship of China


A novel fluorescein derivative, 5-bromosalicylicaldehyde fluorescein hydrazone(BSFH), has been synthesized by reacting fluorescein hydrazine with 5-bromosalicylicaldehyde and characterized by IR, NMR, MS, elemental analysis. The selectivity of BSFH to common metal ions in aqueous solution was investigated by absorption spectroscopy. BSFH has almost no absorption in visible region and very low absorption at 496 nm with addition of other metal ions except Cu2+. However, in the presence of Cu2+, a rapid color change of BSFH from colorless to yellow along with a very obvious absorption appears at 496 nm in the absorption spectrum. The absorbance of BSFH at 496 nm increases gradually with the increase of Cu2+. The selective binding of BSFH to Cu2+ over other metal ions is remarkably high in the aqueous solution. Experimental results indicate that the chemical stoichiometric ratio between BSFH and Cu2+ is 1:1, the linear range of detecting Cu2+ concentration is 0.30~10 μmol/L, common metal ions do not show any interference on the determination of Cu2+, the detection limit is 0.30 μmol/L, and BSFH is highly sensitive to Cu2+ in the aqueous solution. Therefore, BSFH can be utilized to detect Cu2+ at low concentration with simple, rapid and sensitive method.

Key words: fluorescein derivative, bromosalicylicaldehyde fluorescein hydrazone, cupper(Ⅱ) ion, absorption spectrum, detection