电化学发光生物传感技术在快速检测心肌梗死标志物中的研究进展

doi:10.11944/j.issn.1000-0518.2018.09.180170

摘要/Abstract

摘要：

设计和发展简便、高灵敏、高选择性的分析手段以检测低浓度急性心肌梗死生物标志物是目前临床诊断迫切的需求。电化学发光分析法由于具有稳定性好、灵敏度高、线性范围宽及可控性强等优点,能有效地进行低浓度样品检测。该方法与生物传感技术相结合,有利于实现生物体液等复杂样品中极低含量急性心肌梗死生物标志物的快速检测。本文综述了电化学发光生物传感技术在快速检测心肌梗死标志物中近5年的进展,介绍了电化学发光探针和共反应物,以及多组分生物传感检测技术等,并对其在心肌梗死标志物分析中的应用进行了总结。

关键词: 电化学发光, 生物传感器, 电化学发光探针, 阵列, 急性心肌梗死生物标志物分析

Abstract:

Design and development of simple, ultrasensitive, and high selective analytical techniques and methods for the clinical diagnosis and monitoring of a series of acute myocardial infarction(AMI) markers are urgently required for complicated sample matrix. Due to its good stability, high sensitivity, wide linear range, and good controllability, electrochemiluminescent(ECL) analysis technique is commonly used for low-level analytes. Combined with biosensing, it shows great promise in assaying low-level AMI biomarkers in complicated matrix such as biological fluids. This review presents the development of ECL sensing technique for AMI bioanalysis in recent five years. Some ECL probes, co-reactants, multianalyte biosensing methods and their application in AMI marker analysis have been introduced.

Key words: electrochemiluminescence, biosensor, electrochemiluminescence probes, array, acute myocardial infarction markers analysis

覃晓丽,王茗涵,董逸帆,邵元华. 电化学发光生物传感技术在快速检测心肌梗死标志物中的研究进展[J]. 应用化学, 2018, 35(9): 1107-1112.

QIN Xiaoli,WANG Minghan,DONG Yifan,SHAO Yuanhua. Electrochemiluminescent Biosensing and Its Application in Rapid Detection of Acute Myocardial Infarction Markers[J]. Chinese Journal of Applied Chemistry, 2018, 35(9): 1107-1112.

参考文献

[1]	World Health Statistics 2017:Monitoring health for the SDGs, Sustainable Development Goals[R]. Geneva:WHO 2017.
[2]	Han X,Li S,Peng Z,et al. Recent Development of Cardiac Troponin I Detection[J]. ACS Sens,2016,1(2):106-114.
[3]	Arya S K,Bhansali S.Lung Cancer and Its Early Detection Using Biomarker-Based Biosensors[J]. Chem Rev,2011,111(11):6783-6809.
[4]	Kemp M,Donovan J,Higham H,et al. Biochemical Markers of Myocardial Injury[J]. Br J Anaesth,2004,93(1):63-73.
[5]	Pokhrel S,Guotian Y.Microrna and Its Role in Cardiovascular Disease[J]. World J Cardiovasc Dis,2017,7(10):340-357.
[6]	Arya S K,Bhansali S.Lung Cancer and Its Early Detection Using Biomarker-Based Biosensors[J]. Chem Rev,2011,111(11):6783-6809.
[7]	Turner A P F. Biosensors:Sense and Sensibility[J]. Chem Soc Rev,2013,42(8):3184.
[8]	Liu Z,Qi W,Xu G.Recent Advances in Electrochemiluminescence[J]. Chem Soc Rev,2015,44:3117-3142.
[9]	Miao W.Electrogenerated Chemiluminescence and Its Biorelated Applications[J]. Chem Rev,2008,108:2506-2553.
[10]	Harvey N.Luminescence During Electrolysis[J]. J Phys Chem,1928,33(10):1456-1459.
[11]	Miao W,Choi J P,Bard A J.Electrogenerated Chemiluminescence:The Tris(2,2'-Bipyridine) Ruthenium(Ⅱ), ($Ru(bpy)^{2+}_{3}$)/Tri-N-Propylamine(TPrA) System Revisited—A New Route Involving TPrA·+ Cation Radicals[J]. J Am Chem Soc,2002,124:14478-14485.
[12]	Rubinstein I,Bard A J.Electrogenerated Chemiluminescence:Aqueous ECL Systems Based on Tris(2,2'-Bipyridine)Ruthenium2+ and Oxalate or Organic Acids[J]. J Am Chem Soc,1981,103(3):512-516.
[13]	Dick J E,Renault C,Kim B K,et al. Electrogenerated Chemiluminescence of Common Organic Luminophores in Water Using an Emulsion System[J]. J Am Chem Soc,2014,136(39):13546-13549.
[14]	Dick J E,Renault C,Kim B K,et al. Simultaneous Detection of Single Attoliter Droplet Collisions by Electrochemical and Electrogenerated Chemiluminescent Responses[J]. Angew Chem Int Ed,2014,53(44):11859-11862.
[15]	Xu S,Liu Y,Wang T,et al. Positive Potential Operation of a Cathodic Electrogenerated Chemiluminescence Immunosensor Based on Luminol and Graphene for Cancer Biomarker Detection[J]. Anal Chem,2011,83(10):3817-3823.
[16]	Niu H,Yuan R,Chai Y,et al. Highly Enhanced Electrochemiluminescence Based on Synergetic Catalysis Effect of Enzyme and Pd Nanoparticles for Ultrasensitive Immunoassay[J]. Chem Commun,2011,47(29):8397.
[17]	Dong Y,Tian W,Ren S,et al. Graphene Quantum Dots/L-Cysteine Coreactant Electrochemiluminescence System and Its Application in Sensing Lead(Ⅱ) Ions[J]. ACS Appl Mater Interfaces,2014,6:646-1651.
[18]	Jiang D,Du X,Liu Q,et al. One-Step Thermal-Treatment Route to Fabricate Well-Dispersed ZnO Nanocrystals on Nitrogen-Doped Graphene for Enhanced Electrochemiluminescence and Ultrasensitive Detection of Pentachlorophenol[J]. ACS Appl Mater Interfaces,2015,7(5):3093-3100.
[19]	Wang C,Qian J,Wang K,et al. Nitrogen-Doped Graphene Quantum Dots@SiO2 Nanoparticles as Electrochemiluminescence and Fluorescence Signal Indicators for Magnetically Controlled Aptasensor with Dual Detection Channels[J]. ACS Appl Mater Interfaces,2015,7:26865-26873.
[20]	Li L,Chen Y,Zhu J J.Recent Advances in Electrochemiluminescence Analysis[J]. Anal Chem,2017,89(1):358-371.
[21]	Qi H,Chen Y H,Cheng C H,et al. Electrochemistry and Electrogenerated Chemiluminescence of Three Phenanthrene Derivatives, Enhancement of Radical Stability, and Electrogenerated Chemiluminescence Efficiency by Substituent Groups[J]. J Am Chem Soc,2013,135(24):9041-9049.
[22]	Pinaud F,Russo L,Pinet S,et al. Enhanced Electrogenerated Chemiluminescence in Thermoresponsive Microgels[J]. J Am Chem Soc,2013,135(15):5517-5520.
[23]	Li F,Yu Y,Cui H,et al. Label-Free Electrochemiluminescence Immunosensor for Cardiac Troponin I Using Luminol Functionalized Gold Nanoparticles as a Sensing Platform[J]. Analyst,2013,138(6):1844-1850.
[24]	Zhang H,Han Z,Wang X,et al. Sensitive Immunosensor for N-Terminal Pro-brain Natriuretic Peptide Based on N-(Aminobutyl)-N-(Ethylisoluminol)-Functionalized Gold Nanodots/Multiwalled Carbon Nanotube Electrochemiluminescence Nanointerface[J]. ACS Appl Mater Interfaces,2015,7(14):7599-7604.
[25]	Tokel N E,Bard A J.Electrogenerated Chemiluminescence Electrochemistry and Emission from Systems Containing Tris(2,2'-Bipyridine)Ruthenium(Ⅱ) Dichloride[J]. J Am Chem Soc,1972,94(8):2862-2863.
[26]	Qiu R,Zhang X,Luo H,et al. Mass Spectrometric Snapshots for Electrochemical Reactions[J]. Chem Sci,2016,7(11):6684-6688.
[27]	Qi H,Qiu X,Xie D,et al. Ultrasensitive Electrogenerated Chemiluminescence Peptide-Based Method for the Determination of Cardiac Troponin I Incorporating Amplification of Signal Reagent-Encapsulated Liposomes[J]. Anal Chem,2013,85(8):3886-3894.
[28]	Dong M,Li M,Qi H,et al. Electrogenerated Chemiluminescence Peptide-Based Biosensing Method for Cardiac Troponin I Using Peptide-Integrating $Ru(bpy)^{2+}_{3}$-Functionalized Gold Nanoparticles as Nanoprobe[J]. Gold Bull,2015,48:21-29.
[29]	Nasiri Khonsari Y,Sun S.Recent Trends in Electrochemiluminescence Aptasensors and Their Applications[J]. Chem Commun,2017,53(65):9042-9054.
[30]	Shi L,Li X,Zhu W,et al. Sandwich-Type Electrochemiluminescence Sensor for Detection of NT-ProBNP by Using High Efficiency Quench Strategy of Fe3O4@PDA Toward $Ru(bpy)^{2+}_{3}$ Coordinated with Silver Oxalate[J]. ACS Sens,2017,2(12):1774-1778.
[31]	Pur M R K,Hosseini M,Faridbod F,et al. Highly Sensitive Label-Free Electrochemiluminescence Aptasensor for Early Detection of Myoglobin, a Biomarker for Myocardial Infarction[J]. Electrochim Acta,2017,184(9):3529-3537.
[32]	O'Reilly E J,Conroy P J,Hearty S,et al. Electrochemiluminescence Platform for the Detection of C-Reactive Proteins:Application of Recombinant Antibody Technology to Cardiac Biomarker Detection[J]. RSC Adv,2015,5(83):67874-67877.
[33]	Irkham,Watanabe T,Fiorani A,et al. Co-Reactant-on-Demand ECL:Electrogenerated Chemiluminescence by the in Situ Production of $S_{2}O^{2-}_{8}$ at Boron-Doped Diamond Electrodes[J]. J Am Chem Soc,2016,138(48):15636-15641.
[34]	Long Y M,Bao L,Zhao J Y,et al. Revealing Carbon Nanodots as Coreactants of the Anodic Electrochemiluminescence of $Ru(bpy)^{2+}_{3}$[J]. Anal Chem,2014,86(15):7224-7228.
[35]	Venkateswara Raju C,Senthil Kumar S.Highly Sensitive Novel Cathodic Electrochemiluminescence of Tris(2,2'-Bipyridine) Ruthenium(Ⅱ) Using Glutathione as a Co-reactant[J]. Chem Commun,2017,53(49):6593-6596.
[36]	Qin X,Gu C,Wang M,et al. Triethanolamine-Modified Gold Nanoparticles Synthesized by a One-Pot Method and Their Application in Electrochemiluminescent Immunoassy[J]. Anal Chem,2018,90(4):2826-2832.
[37]	Wu P,Hou X,Xu J J,et al. Ratiometric Fluorescence, Electrochemiluminescence, and Photoelectrochemical Chemo/Biosensing Based on Semiconductor Quantum Dots[J]. Nanoscale,2016,8(16):8427-8442.
[38]	Yang X,Yu Y Q,Peng L Z,et al. Strong Electrochemiluminescence from MOF Accelerator Enriched Quantum Dots for Enhanced Sensing of Trace cTnI[J]. Anal Chem,2018,90(6):3995-4002.
[39]	Zheng H,Zhang Q,Hong Z,et al. A Bifunctional Catalyst Based ECL Immunosensor for a Cardiac Biomarker Regulated by Oxygen Evolution Reaction[J]. Electrochim Acta,2016,215:326-333.
[40]	Bist I,Song B,Mosa I M,et al. Electrochemiluminescent Array to Detect Oxidative Damage in ds-DNA Using [Os(bpy)2(Phen-Benz-COOH)]2+/Nafion/Graphene Films[J]. ACS Sens,2016,1(3):272-278.
[41]	Qi W,Lai J,Gao W,et al. Wireless Electrochemiluminescence with Disposable Minidevice[J]. Anal Chem,2014,86(18):8927-8931.
[42]	Zhang H R,Wang Y Z,Zhao W,et al. Visual Color-Switch Electrochemiluminescence Biosensing of Cancer Cell Based on Multichannel Bipolar Electrode Chip[J]. Anal Chem,2016,88(5):2884-2890.
[43]	Yang X,Zhao Y,Sun L,et al. Electrogenerated Chemiluminescence Biosensor Array for the Detection of Multiple Ami Biomarkers[J]. Sens Actuators B,2018,257:60-67.
[44]	Liang W,Fan C,Zhuo Y,et al. Multiparameter Analysis-Based Electrochemiluminescent Assay for Simultaneous Detection of Multiple Biomarker Proteins on a Single Interface[J]. Anal Chem,2016,88(9):4940-4948.