Nafion-碳纳米管修饰电极电化学发光分析法测定卡马西平

doi:10.19894/j.issn.1000-0518.200251

摘要/Abstract

摘要： 建立了一种利用修饰电极检测卡马西平含量的电化学发光新方法。首先通过滴涂与离子交换法制备了Nafion-碳纳米管(CNTs)/Ru(bpy)²⁺₃玻碳修饰电极,并利用循环伏安法与电化学发光信号-电势图谱对其进行了初步表征,同时初步探讨了该方法检测卡马西平含量的可能原理;再基于中性介质中,少量卡马西平能显著增敏Ru(bpy)²⁺₃的电化学发光信号,利用增敏的电化学发光强度对卡马西平进行分析检测。在优化的实验条件下,卡马西平浓度在3.0×10^-9～1.0×10^-7 mol/L浓度范围内与增敏的电化学发光信号呈现良好的线性相关,检出限与相对标准偏差分别为1.5×10^-9 mol/L及1.9%(c=1.0×10^-8 mol/L,n=11)。应用该方法测定片剂卡马西平含量,平均回收率为102.3%,结果较为满意,有望应用于临床检验中卡马西平含量的准确分析。

关键词: Nafion/碳纳米管, 三( 2,2'-联吡啶)钌(II), 修饰电极, 电化学发光, 卡马西平

Abstract: A novel electrochemiluminescence (ECL) method with the modified electrode for detecting carbamazepine was established. Firstly, a Nafion-Carbon nanotubes (CNTs)/Ru(bpy)²⁺₃ modified electrode (Nafion-CNT/Ru(bpy)²⁺₃/GCE) was prepared by coating and ion exchange strategy, and was primarily characterized by cyclic voltammetric method and ECL-potential spectrograms. Moreover, this work preliminarily discusses the possible principle for the determination of carbamazepine. In the neutral medium, it is found that a small amount of carbamazepine could significantly heighten the ECL signal of Ru(bpy)²⁺₃. By enhancing the ECL signal, the content of carbamazepine can be quantitatively determined. Under the optimal conditions, a good linear relationship between the enhancing ECL signal and carbamazepine concentration from 3.0×10^-9 to 1.0×10^-7 mol/L is obtained. The detection limit and relative standard deviation (RSD) are 1.5×10^-9 mol/L and 1.9% (c=1.0×10^-8 mol/L, n=11), respectively. This method is applied to the detection of carbamazepine in tablets with an average recovery of 102.3%. The results are more satisfactory and the proposed method can be applied to accurate analysis of carbamazepine content in clinical examination.

Key words: Nafion-Carbon nanotubes, Tris(2,2'-bipyridine)ruthenium(Ⅱ), Modified electrode, Electrochemiluminescence, Carbamazepine

中图分类号:

O657.1

熊海涛, 吴睿, 吴迎春. Nafion-碳纳米管修饰电极电化学发光分析法测定卡马西平[J]. 应用化学, 2021, 38(6): 731-738.

XIONG Hai-Tao, WU Rui, WU Ying-Chun. Electrochemiluminescence Determination of Carbamazepine Based on the Nafion-Carbon Nanotube Modified Electrode[J]. Chinese Journal of Applied Chemistry, 2021, 38(6): 731-738.

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