应用化学 ›› 2019, Vol. 36 ›› Issue (5): 595-602.DOI: 10.11944/j.issn.1000-0518.2019.05.180310

• 研究论文 • 上一篇    下一篇

基于主客体识别作用构建的电化学发光传感器检测丹参中汞离子

韦国兵,孔德荣,徐慧慧,殷赵江,张晶,崔汉峰,洪年,杨婕,熊魏,刘文明,郭倩文,程林(),樊浩()   

  1. 江西中医药大学药学院 南昌 330004
  • 收稿日期:2018-09-20 接受日期:2019-01-23 出版日期:2019-05-01 发布日期:2019-05-06
  • 通讯作者: 程林,樊浩
  • 基金资助:
    国家自然基金(81660658,81860682,81560625,81872968),江西省青年自然科学基金(20161BAB215212),江西省卫计委中医药计划(2017A280),江西中医药大学校级研究生创新专项资金项目(JZYC17S07,JZYC18S13),江西中医药大学重点学科支持计划(2017jzzdxk036),江西省教育厅科技项目(GJJ160833)资助

A New Type of Electrochemiluminescence Sensor for Detection of Mercury Ion in Chinese Medicinal Materials Danshen Based on Host-Guest Interaction

WEI Guobing,KONG Derong,XU Huihui,YIN Zhaojiang,ZHANG Jing,CUI Hanfeng,HONG Nian,YANG Jie,XIONG Wei,LIU Wenming,GUO Qianwen,CHENG Lin(),FAN Hao()   

  1. Department of Pharmacy,Jiangxi University of Traditional Chinese Medicine,Nanchang 330004,China
  • Received:2018-09-20 Accepted:2019-01-23 Published:2019-05-01 Online:2019-05-06
  • Contact: CHENG Lin,FAN Hao
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.81660658, No.81860682, No.81560625, No.81872968), Jiangxi Youth Natural Science Foundation(No.20161BAB215212), Traditional Chinese Medicine Program of Jiangxi Provincial Health Planning Commission(No.2017A280), Innovation Project for Graduate Students of Jiangxi University of Traditional Chinese Medicine(No.JZYC17S07, No.JZYC18S13), Key Subject Support Program of Jiangxi University of Traditional Chinese Medicine(No.2017jzzdxk036), Science and Technology Project of Jiangxi Education Department(No.GJJ160833)

摘要:

使用联吡啶钌(Ru(bpy)32+)/β-环糊精-金纳米粒子(β-CD-AuNPs)/全氟磺酸(Nafion)复合物和二茂铁标记(Fc)的DNA探针(Fc-DNA)构建了电化学发光(Electrochemiluminescence,ECL)生物传感器,将其用于检测中药材丹参中的汞离子。 该传感器包含ECL发光基底和ECL强度开关两部分,将Ru(bpy)32+/β-CD-AuNPs/Nafion组装到玻碳电极(GCE)上构成发光基底,产生稳定的ECL信号;Fc-DNA探针作为ECL信号开关,通过分子识别策略设计,利用β-CD与Fc的主客体相互作用与β-CD-AuNPs相连。 该检测方法和以往的汞离子检测方法相比,具有背景信号低、选择性高、仪器简单、操作快速等特点。 该 “Off-On” 电致化学发光生物传感器在0.04~800 ng/mL范围内对Hg2+具有良好的线性响应,检测限为0.02 ng/mL(S/N=3)。

关键词: 电致化学发光, 生物传感器, 汞离子, 分子识别技术, 开关

Abstract:

A new type of “Off-On” electrochemiluminescence(ECL) biosensor using Ru(bpy)32+/β-CD-AuNPs/Nafion complex and ferrocene-labeled DNA(Fc-DNA) was designed to detect mercury ions in Chinese medicinal materials Danshen. The ECL biosensor includes an ECL substrate and an ECL signal switch. Ru(bpy)32+/β-CD-AuNPs/Nafion complex modified glassy carbon electrode was used as the ECL substrate, which could bring about a clear and stable ECL signal by Ru(bpy)32+, and hairpin-like Fc-DNA probe acted as the ECL signal switch, which was designed by molecular recognition strategy and attached to β-CD-AuNPs through host-guest interaction between β-CD and ferrocene . Compared with previous methods of mercury ion detection, this method has the advantages of convenient operation, high sensitivity, good repeatability and low detection limit. The ECL biosensor offered good linear responses for Hg2+ in the range of 0.04~800 ng/mL with a detection limit of 0.02 ng/mL(S/N=3).

Key words: electrochemiluminescence, biosensor, mercury(Ⅱ), molecular recognition technology, Switch