血红素/G-四链体DNA酶的活性增强研究及其在生物传感器中的应用进展

doi:10.11944/j.issn.1000-0518.2020.11.200153

摘要/Abstract

摘要： 血红素/G-四链体DNA酶是一类具有类过氧化物酶活性的DNA分子,因其具有出色的活性、易修饰性和可编程性,被广泛应用于生物传感器等领域。本文先是简要介绍了G-四链体的结构,再主要综述了增强血红素/G-四链体DNA酶活性的策略及基于血红素/G-四链体DNA酶的生物传感器在生物标志物、微生物与生物毒素以及金属离子检测中的应用,并展望了血红素/G-四链体DNA酶的未来发展趋势。

关键词: 血红素/G-四链体, DNA酶, 过氧化物酶, 生物传感器

Abstract: Hemin/G-quadruplex DNAzyme is a type of DNA molecule with peroxidase-like activity. Because of its excellent activity, easy modification and programmability, it has been widely used in biosensors and other fields. This article first briefly introduces the structure of G-quadruplex, and then reviews the strategies for enhancing hemin/G-quadruplex DNAzyme activity and application of biosensors based on hemin/G-quadruplex DNAzyme in the detection of biomarkers, microorganisms, biotoxins and metal ions. We also prospect the development trend of hemin/G-quadruplex DNAzyme.

Key words: hemin/G-quadruplex, dnazyme, peroxidase, biosensor

中图分类号:

O657

莫艳红, 李晖, 王彬, 徐晓慧, 刘思思, 曾冬冬. 血红素/G-四链体DNA酶的活性增强研究及其在生物传感器中的应用进展[J]. 应用化学, 2020, 37(11): 1249-1261.

MO Yanhong, LI Hui, WANG Bin, XU Xiaohui, LIU Sisi, ZENG Dongdong. Research on the Enhanced Activity of Hemin/G-Quadruplex DNAzyme and Its Application in Biosensors[J]. Chinese Journal of Applied Chemistry, 2020, 37(11): 1249-1261.

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