应用化学 ›› 2020, Vol. 37 ›› Issue (10): 1211-1220.DOI: 10.11944/j.issn.1000-0518.2020.10.200064

• 研究论文 • 上一篇    

具有过氧化物酶活性的Imm-Fe3+-IL的制备及用于比色法测定H2O2和葡萄糖

喻昌木a, 张荣b, 卢小鸾a, 杨敏a*, 彭黔荣c*   

  1. a贵州大学药学院 贵阳 550025;
    b贵州大学化学与化工学院 贵阳 550025;
    c贵州中烟工业有限责任公司技术中心 贵阳 550009
  • 收稿日期:2020-03-12 出版日期:2020-10-01 发布日期:2020-09-30
  • 通讯作者: 杨敏,教授; Tel:0851-85588075; E-mail:2578973180@qq.com; 研究方向:催化材料的合成与应用
    共同通讯联系人:彭黔荣,副教授; Tel:0851-83981462; E-mail:3435391@qq.com; 研究方向:烟草化学与工程
  • 基金资助:
    国家自然科学基金资助(21562014)、贵州中烟科技(黔烟工技[2015]8号)项目

Preparation of Imm-Fe3+-IL with Peroxidase Activity and Its Colorimetric Determination of H2O2 and Glucose

YU Changmua, ZHANG Rongb, LU Xiaoluana, YANG Mina*, PENG Qianrongc*   

  1. aSchool of Pharmacy,Guizhou University,Guiyang 550025,China;
    bCollege of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550025,China;
    cTechnology Center of Guizhou China Tobacco Industry Co.,Ltd,Guiyang 550009,China
  • Received:2020-03-12 Published:2020-10-01 Online:2020-09-30
  • Contact: YANG Min, professor; Tel:0851-85588075; E-mail:2578973180@qq.com; Research interests:synthesis and application of catalytic materials
    Co-corresponding author:PENG Qianrong, associate professor; Tel:0851-83981462; E-mail:3435391@qq.com; Research interests:tobacco chemistry and engineering
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.21562014), Guizhou Zhongyan Technology Project(Qianyan Gongji [2015] No.8)

摘要: 通过溶胶-凝胶法制备的Imm-Fe3+-IL纳米材料具有类过氧化物酶的活性,能够催化过氧化氢(H2O2)快速氧化3,3′,5,5′-四甲基联苯胺(TMB)产生相应的颜色变化。 稳态动力学分析表明,Imm-Fe3+-IL纳米材料遵循典型的Michaelis-Menten模型和乒乓机理。 辣根过氧化物酶(HRP)相比,Imm-Fe3+-IL纳米材料纳米材料具有更强的亲和性。 联合葡萄糖氧化酶建立了H2O2和葡萄糖的比色检测方法。 结果显示:H2O2和葡萄糖的浓度与反应体系的吸光度呈良好的线性关系,H2O2的线性范围为1~200 μmol/L,葡萄糖的线性范围为10~200 μmol/L,最低检出限(LOD)分别为0.35和3.31 μmol/L。

关键词: 离子液体, 过氧化物酶, 比色检测, H2O2, 葡萄糖

Abstract: The Imm-Fe3+-IL nanomaterials prepared by the sol-gel method have peroxidase-like activity,which can catalyze the rapid oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) with hydrogen peroxide (H2O2) to produce corresponding color changes. The steady-state kinetic analysis shows that the catalytic kinetics of Imm-Fe3+-IL follows the typical Michaelis-Menten model, and the Ping-Pong mechanism. Compared with horseradish peroxidase (HRP), Imm-Fe3+-IL nanomaterials have stronger affinity. Combined with glucose oxidase, a colorimetric detection method for H2O2 and glucose is established. The results show that the concentration of H2O2 and glucose shows a good linear relationship with the absorbance of the reaction system. The linear range of H2O2 is 1~200 μmol/L, the linear range of glucose is 10~200 μmol/L, and the limits of detection (LOD) are 0.35 and 3.31 μmol/L for H2O2 and glucose, respectively.

Key words: ionic liquid, peroxidase-like, colorimetric detection, H2O2, glucose