基于微悬臂梁生物传感器检测三磷酸腺苷

doi:10.11944/j.issn.1000-0518.2015.03.140437

摘要/Abstract

摘要：

基于适配子构建了无标记检测三磷酸腺苷(ATP)的微悬臂梁生物传感器。将ATP适配子修饰在微悬臂梁阵列中的传感悬臂镀金面上,用来识别ATP,而参比悬臂修饰巯基己醇(MCH)防止非特异性吸附。 ATP与其适配子发生特异性相互作用,使悬臂的上下两个表面产生应力差,导致传感悬臂产生偏转,扣除参比悬臂偏转后其偏转值与ATP的浓度在0.5~5 mmol/L范围内有良好的线性关系,相关系数为0.998,最低检出限为0.06 mmol/L。该微悬臂梁生物传感器响应快速、操作简单,并且对ATP具有良好的特异性。

关键词: 三磷酸腺苷, 适配子, 微悬臂梁生物传感器, 表面应力

Abstract:

An aptamer-based microcantilever biosensor for label-free detection of adenosine triphosphate(ATP) was developed. The sensor cantilevers in the microcantilever array were functionalized with ATP aptamer on the gold side to recognize ATP, while the reference cantilevers were modified by 6-mercapto-1-hexanol(MCH) to prevent non-specific absorption. The interaction between ATP and aptamer on the sensor cantilerers gold surface induced surface stress changes, resulting in a differential deflection between the sensor and reference cantilevers. The differential deflection has a good linear relationship with the ATP concentration ranging from 0.5~5 mmol/L with a correlation coefficient of 0.998. The detection limit is about 0.06 mmol/L. The aptamer-based microcantilever sensor shows rapid response, simple procedures and high specificity for ATP.

Key words: adenosine triphosphate, aptamer, microcantilever biosensor, surface stress

中图分类号:

李辉彦, 白效静, 王风红, 李静, 唐纪琳. 基于微悬臂梁生物传感器检测三磷酸腺苷[J]. 应用化学, 2015, 32(3): 362-366.

LI Huiyan, BAI Xiaojing, WANG Fenghong, LI Jing, TANG Jilin. Microcantilever Biosensor for Detection of Adenosine Triphosphate[J]. Chinese Journal of Applied Chemistry, 2015, 32(3): 362-366.

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