基于媒介体的电化学水体总毒性检测方法现状及展望

doi:10.11944/j.issn.1000-0518.2018.09.180136

摘要/Abstract

摘要：

基于媒介体的电化学水体总毒性检测方法是一种灵敏、可靠的水体总毒性检测方法。它以原位培养微生物为毒性检测受试体,能够更加准确的反映目标水体的水体总毒性变化,因此适合用于水体复合污染的评估及水质安全预警。本文从检测原理、媒介体、微生物等方面详细阐述了该方法的研究现状,并对该方法今后的发展方向进行了展望。

关键词: 媒介体, 电化学, 水体总毒性, 复合污染, 水质预警

Abstract:

The mediator-based electrochemical method is a sensitive and reliable one toward water total toxicity. The in-situ cultivated microorganism can be used as the test organisms, which endows the method high sensitivity to the changes in the total toxicity of the target water, thus enabling the method suitable for the evolution of the exact level of complex pollution of water and the design of early warning system of water quality. Here, the advance of the mediator-based electrochemical method toward water total toxicity is reviewed in term of mechanism of toxicity determination, the use of mediator and microorganism, and so on. Finally, a future perspective of the method is presented.

Key words: mediator, electrochemistry, water total toxicity, complex pollution, early warning of water quality

翟俊峰,余登斌,刘玲,董绍俊. 基于媒介体的电化学水体总毒性检测方法现状及展望[J]. 应用化学, 2018, 35(9): 1102-1106.

ZHAI Junfeng,YU Dengbin,LIU Ling,DONG Shaojun. Advance and Future Development of Mediator-Based Electrochemical Method Toward Water Total Toxicity[J]. Chinese Journal of Applied Chemistry, 2018, 35(9): 1102-1106.

参考文献

[1]	Cho J C,Park K J,Ihm H S,et al. A Novel Continuous Toxicity Test System Using a Luminously Modified Freshwater Bacterium[J]. Biosens Bioelectron,2004,20(2):338-344.
[2]	Bock Gu M,Cheol Gil G.A Multi-Channel Continuous Toxicity Monitoring System Using Recombinant Bioluminescent Bacteria for Classification of Toxicity[J]. Biosens Bioelectron,2001,16(9/12):661-666.
[3]	Gu M B,Gil G C,Kim J H.A Two-Stage Minibioreactor System for Continuous Toxicity Monitoring[J]. Biosens Bioelectron,1999,14(4):355-361.
[4]	Tizzard A,Webber J,Gooneratne R,et al. MICREDOX:Application for Rapid Biotoxicity Assessment[J]. Anal Chim Acta,2004,522(2):197-205.
[5]	ZHAI Junfeng,YU Dengbin,DONG Shaojun.An Online Equipment for Monitoring of Water Total Toxicity[J]. Chinese J Anal Chem,2017,45(9):1415-1419(in Chinese). 翟俊峰,余登斌,董绍俊. 水体总毒性在线检测仪器的研制[J]. 分析化学,2017,45(9):1415-1419.
[6]	Fang D,Gao G,Shen J,et al. A Reagentless Electrochemical Biosensor Based on Thionine Wrapped E-Coli and Chitosan-Entrapped Carbon Nanodots Film Modified Glassy Carbon Electrode for Wastewater Toxicity Assessment[J]. Electrochim Acta,2016,222:303-311.
[7]	Liu C,Sun T,Xu X,et al. Direct Toxicity Assessment of Toxic Chemicals with Electrochemical Method[J]. Anal Chim Acta,2009,641(1/2):59-63.
[8]	Liu C,Sun T,Zhai Y,et al. Evaluation of Ferricyanide Effects on Microorganisms with Multi-Methods[J]. Talanta,2009,78(2):613-617.
[9]	Yu D,Zhai J,Yong D,et al. A Rapid and Sensitive p-Benzoquinone-mediated Bioassay for Determination of Heavy Metal Toxicity in Water[J]. Analyst,2013,138(11):3297-3302.
[10]	Wang X,Liu M,Wang X,et al. P-Benzoquinone-Mediated Amperometric Biosensor Developed with Psychrobacter sp. for Toxicity Testing of Heavy Metals[J]. Biosens Bioelectron,2013,41:557-562.
[11]	Pasco N F,Goonerate R,Daniel R M,et al. Toxicity Assessment of Chlorophenols Using a Mediated Microbial Toxicity Assay[J]. Int J Environ Anal Chem,2008,88(15):1063-1075.
[12]	Zhao J,Wang Z,Wang M,et al. The Interaction Mechanisms Between Saccharomyces Cerevisiae and Menadione and Its Application in Toxicology Study[J]. Talanta,2008,74(5):1686-1691.
[13]	Rawson F J,Downard A J,Baronian K H.Electrochemical Detection of Intracellular and Cell Membrane Redox Systems in Saccharomyces Cerevisiae[J]. Sci Rep,2014,4(6):5216.
[14]	Gao G,Fang D,Yu Y,et al. A Double-Mediator Based Whole Cell Electrochemical Biosensor for Acute Biotoxicity Assessment of Wastewater[J]. Talanta,2017,167:208-216.
[15]	Wang M,Wang Z Q,Li S,et al. Mediated Electrochemical Method for the Analysis of Membrane Damage Effects of Phenolic Compounds to Staphylococcus Aureus[J]. J Electroanal Chem,2015,757:44-50.
[16]	Liu C,Yong D,Yu D,et al. Cell-Based Biosensor for Measurement of Phenol and Nitrophenols Toxicity[J]. Talanta,2011,84(3):766-770.
[17]	Jordan M A,Welsh D T,Teasdale P R,et al. A Ferricyanide-Mediated Activated Sludge Bioassay for Fast Determination of the Biochemical Oxygen Demand of Wastewaters[J]. Water Res,2010,44(20):5981-5988.
[18]	Ma H P,Yong D M,Kim H J,et al. A Ferricyanide-Mediated Activated Sludge Bioassay for Determination of the Toxicity of Water[J]. Electroanalysis,2016,28(3):580-587.
[19]	Yong D,Liu L,Yu D,et al. Development of a Simple Method for Biotoxicity Measurement Using Ultramicroelectrode Array Under Non-Deaerated Condition[J]. Anal Chim Acta,2011,701(2):164-168.
[20]	Li J M,Yu Y,Qian J,et al. A Novel Integrated Biosensor Based on Co-Immobilizing the Mediator and Microorganism for Water Biotoxicity Assay[J]. Analyst,2014,139(11):2806-2812.
[21]	Teitzel G M,Parsek M R.Heavy Metal Resistance of Biofilm and Planktonic Pseudomonas aeruginosa[J]. Appl Environ Microbiol,2003,69(4):2313-2320.
[22]	Harrison J J,Ceri H,Stremick C A,et al. Biofilm Susceptibility to Metal Toxicity[J]. Environ Microbiol,2004,6(12):1220-1227.
[23]	Ricco G,Tomei M C,Ramadori R,et al. Toxicity Assessment of Common Xenobiotic Compounds on Municipal Activated Sludge:Comparison Between Respirometry and Microtox[J]. Water Res,2004,38(8):2103-2110.
[24]	Gao G Y,Qian J,Fang D Y,et al. Development of a Mediated Whole Cell-Based Electrochemical Biosensor for Joint Toxicity Assessment of Multi-Pollutants Using a Mixed Microbial Consortium[J]. Anal Chim Acta,2016,924:21-28.
[25]	Qian J,Li J M,Fang D Y,et al. A Disposable Biofilm-Modified Amperometric Biosensor for the Sensitive Determination of Pesticide Biotoxicity in Water[J]. RSC Adv,2014,4(98):55473-55482.
[26]	Wang H,Wang X J,Zhao J F,et al. Toxicity Assessment of Heavy Metals and Organic Compounds Using Cell Sense Biosensor with E.Coli[J]. Chinese Chem Lett,2008,19(2):211-214.
[27]	Catterall K,Robertson D,Hudson S,et al. A Sensitive, Rapid Ferricyanide-Mediated Toxicity Bioassay Developed Using Escherichia Coli[J]. Talanta,2010,82(2):751-757.
[28]	Yong D,Liu C,Yu D,et al. A Sensitive, Rapid and Inexpensive Way to Assay Pesticide Toxicity Based on Electrochemical Biosensor[J]. Talanta,2011,84(1):7-12.
[29]	Yang Y,Fang D,Liu Y,et al. Problems Analysis and New Fabrication Strategies of Mediated Electrochemical Biosensors for Wastewater Toxicity Assessment[J]. Biosens Bioelectron,2018,108:82-88.