异硫氰酸荧光素标记万古霉素的制备及其与细菌相互作用的荧光检测

doi:10.3724/SP.J.1095.2013.30114

摘要/Abstract

摘要： 以异硫氰酸荧光素（FITC）标记万古霉素（Vanco）制备了FITCVanco（FV），FV经高效液相制备色谱纯化后，进行了质谱结构鉴定。分别通过荧光分光光度计和流式细胞仪（FCM）测定不同浓度FV培养基中培养细菌细胞的荧光强度。结果显示，所制备的荧光探针对5种细菌有特异性结合，用荧光强度可表征细胞结合抗生素的数量，对细菌耐药机制研究和细菌对万古霉素作用敏感性的测试有应用价值。

关键词: 万古霉素, 异硫氰酸荧光素, 荧光探针, 细菌检测

Abstract: Fluorescein isothiocyanate(FITC)-labeled vancomycin(FITC-Vanco, FV) was synthesized. After purified by preparative HPLC, the structure of the target compound was indentified by MS. Its combination amount toward bacteria with different concentration was evaluated by the fluorescence intensity of the bacterial suspension. FV synthesized in this study shows specific binding with different bacteria. It shows promising application value in studying bacterial resistance mechanism and bacterial susceptibility to vancomycin.

中图分类号:

O641

徐蓉, 阮林高, 戈梅, 张怡轩. 异硫氰酸荧光素标记万古霉素的制备及其与细菌相互作用的荧光检测[J]. 应用化学, DOI: 10.3724/SP.J.1095.2013.30114.

XU Rong1, RUAN Lingao2, GE Mei3, ZHANG Yixuan1*. Synthesis of Fluorescein Isothiocyanate-labeled Vancomycin and Its Bacterial Detection by Fluorescence[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2013.30114.

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