鲁米诺-过氧化氢-纳米银化学发光体系测定药物中的对乙酰氨基酚

doi:10.3724/SP.J.1095.2013.30065

摘要/Abstract

摘要： 碱性条件下，对乙酰氨基酚对鲁米诺-过氧化氢-纳米银化学发光体系有较强的抑制作用，基于此，结合流动注射技术，建立了测定对乙酰氨基酚的新方法。研究了影响化学发光强度的各种因素，并初步探讨了可能的发光机理。在最佳实验条件下，对乙酰氨基酚浓度在2.0×10-8～1.0×10-4 mol/L范围内与相对发光强度呈线性关系，检出限(3σ)为4.0×10-9 mol/L。对1.0×10-7 mol/L的对乙酰氨基酚平行测定9次，相对标准偏差为2.6%。该法用于片剂中扑热息痛含量的测定，结果令人满意。

关键词: 化学发光, 纳米银, 鲁米诺, 对乙酰氨基酚

Abstract: A novel method for the determination of acetaminophen was developed based on its inhibition effect on the luminol-H2O2-silver nanoparticle chemiluminescence system. Various factors influencing the chemiluminescence system were investigated and the possible mechanism was also studied. Under the optimum conditions, the linear range for the determination of acetaminophen is 2.0×10-8 to 1.0×10-4 mol/L with a detection limit of 4.0×10-9 mol/L. The relative standard deviation is 2.6% for 1.0× 10-7 mol/L acetaminophen solution in nine repeated measurements. The method was successfully applied to the determination of acetaminophen in pharmaceutical.

Key words: chemiluminescence, Ag nanoparticles, luminol, acetaminophen

中图分类号:

O657.2

樊雪梅, 王书民, 崔效伟. 鲁米诺-过氧化氢-纳米银化学发光体系测定药物中的对乙酰氨基酚[J]. 应用化学, DOI: 10.3724/SP.J.1095.2013.30065.

FAN Xuemei1,2*, WANG Shumin1, CUI Xiaowei1. Determination of Acetaminophen in Pharmaceuticals by Luminol-H2O2-Silver Nanoparticle Chemiluminescence System[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2013.30065.

参考文献

[1] Bessems J G,Vermeulen N P. Paracetamol(acetaminophen) Induced Toxicity:Molecular and Biochemical Mechanisms, Analogues and Protective Approaches[J]. Crit Rev Toxicol,2001,31(1):55-138.

[2] Srivastava M K,Ahmad S,Singh D,et al. Titrimetric Determination of Dipyrone and Paracetamol with Potassium Hexacyanoferrate(Ⅲ) in an Acidic Medium[J]. Analyst,1985,110(6):735-737.

[3] Ni Y N,Li C,Kokot S. Simultaneous Kinetic Spectrophotometric Determination of Acetaminophen and Phenobarbital by Artificial Neural Networks and Partial Least Squares[J]. Anal Chim Acta,2000,419(2):185-196.

[4] Afshari J T,Liu T Z. Rapid Spectrophotometric Method for the Quantitation of Acetaminophen in Serum[J]. Anal Chim Acta,2001,443(1):165-169.

[5] Murillo P J A,Bermejo L F G. Flow-injection Stopped-flow Spectrofluorimetric Kinetic Determination of Paracetamol Based on Its Oxidation Reaction by Hexacyanoferrate(Ⅲ)[J]. Anal Chim Acta,1996,333(1/2):59-69.

[6] MA Xinying,WU Yifang,LI Xia. Preparation of Graphene Modifiedelectrode and Volt-ampere Determination of Acetaminophen[J]. Chinese J Appl Chem,2012,29(7):824-829(in Chinese).

马心英,吴义芳,李霞.石墨烯修饰电极的制备及其对对乙酰氨基酚的伏安测定[J]. 应用化学,2012,29(7):824-829.

[7] Meyer J,Karst U. Determination of Paracetamol(acetaminophen) by HPLC with Post-column Enzymatic Derivatization and Fluorescence Detection[J]. Chromatographia,2001,54(10):163-168.

[8] Satínsk D,Neto I,Solich P,et al. Sequential Injection Chromatographic Determination of Paracetamol, Caffeine, and Acetylsalicylic Acid in Pharmaceutical Tablets[J]. J Sep Sci,2004,27(7/8):529-536.

[9] Fan X M,Wang S M,Su Z K,et al. Determination of Cinnamic Acid in Human Urine by Flow Injection Chemiluminescence[J]. Quimica Nova,2011,34(8):1405-1408.

[10] Ruengsitagoon W,Liawruangrath S,Townshend A. Flow Injection Chemiluminescence Determination of Paracetamol[J]. Talanta,2006:976-983.

[11] Koukli T,Calokerinos A C,Hadjiioannou T P. Continuous-flow Chemiluminescence Determination of Acetaminophen by Reduction of Cerium(Ⅳ)[J]. Analyst,1989,114(6):711-714.

[12] Alapont A G,Zamora L L,Calatayud J M. Indirect Determination of Paracetamol in Pharmaceutical Formulations by Inhibition of the System Luminol-H2O2 Fe(CN)3-6 Chemiluminescence[J]. J Pharm Biomed Anal,1999,21(2):311-317.

[13] Easwaramoorthy D,Yu Y C,Huang H J. Chemiluminescence Detection of Paracetamol by a Luminol-permanganate Based Reaction[J]. Anal Chim Acta,2001,439(1):95-100.

[14] FAN Xuemei,WANG Shumin,SUI Zhikui,et al. FI-Chemiluminescence Determination of Paracetamol[J]. Phys Test Chem Anal Part B:Chem Anal,2011,47(10):1149-1151(in Chinese).

樊雪梅,王书民,苏智魁,等流动注射化学发光法测定扑热息痛[J]. 理化检验:(化学分册),2011,47(10):1149-1151.

[15] WANG Shumin,FAN Xuemei,SUI Zhikui,et al. Flow Injection Chemiluminescence Determination of Paracetamol with Chromium(Ⅵ)-H2O2-luminol System[J]. Chinese J Anal Lab,2011,30(4):120-122(in Chinese).

王书民,樊雪梅,苏智魁,等偶合反应流动注射化学发光法测定扑热息痛[J]. 分析试验室,2011,30(4):120-122.

[16] XIONG Xunyu,TANG Yuhai,WANG Nannan,et al. Determination of Paracetamol by Flow-Injection Chemiluminescence with KMnO4-Na2SO3 System[J]. Chinese J Anal Lab,2007,26(2):80-83(in Chinese).

熊迅宇,唐玉海,王楠楠,等KMnO4-Na2SO3化学发光体系测定对乙酰氨基酚[J]. 分析试验室,2007,26(2):80-83.

[17] FAN Xuemei,WANG Shumin,SUI Zhikui,et al. Determination of Paracetamol in Pharmaceuticals by Fe3+-H2O2-Dichlorofluorescein Chemiluminescence System[J]. J Instrum Anal,2012,31(7):873-876(in Chinese).

樊雪梅,王书民,苏智魁,等 Fe3+-H2O2-二氯荧光素化学发光体系测定药物中的扑热息痛[J]. 分析测试学报,2012,31(7):873-876.

[18] Guo J Z,Cui H,Zhou W,et al. Ag Nanoparticle-catalyzed Chemiluminescent Reaction Between Luminol and Hydrogen Peroxide[J]. J Photochem Photobiol A,2008,193(2/3):89-92.

[19] Editorial Committee of Pharmacopoeia of Hygiene Department of People′ s Republic of China. The Pharmacopoeia of People′ s Republic of China(Part 2). Beijing:Chemical Industry Press & Guangdong Science and Technology Press,1995:196(in Chinese).

中华人民共和国卫生部药典编委会. 中华人民共和国药典（第二部）. 北京：化学工业及广东科学技术出版社，1995:196

[20] Stewart S,Fredericks P M. Surface-enhanced Reman Spectroscopy of Amino Acids Adsorbed on an Electrochemically Prepared Silver[J]. Spectrochim Acta Part A,1999,55(13):641-645.