应用化学

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光谱和电化学方法研究黄芩素与核酸相互作用的机理

郭蒙1,2*,王淑敏2,王佳丽2,李丹2,高庆宇1*,李丽敏2   

  1. (1.中国矿业大学化工学院 徐州 221116;2.潍坊学院化学化工与环境工程学院 潍坊 261061)
  • 收稿日期:2013-05-13 修回日期:2013-06-07 出版日期:2014-01-10 发布日期:2014-01-10
  • 通讯作者: 郭蒙,高工; Tel:0536-8785283; E-mail:guomeng7101@126.com; 研究方向:光谱电化学分析及应用、生物学
  • 基金资助:
    山东省自然科学基金资助项目(Y2008B30),潍坊市科技发展计划项目(2060402)

Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA

GUO Meng1,2*, WANG Shumin2, WANG Jiali2, LI Dan2, GAO Qingyu1*, LI Limin2   

  1. (1.School of Chemical Engineering and Technology,China University of Mining and Technology,Xuzhou 221116,China;
    2.Chemistry & Chemical and Environmental Engineering College,Weifang University,Weifang 261061,China)
  • Received:2013-05-13 Revised:2013-06-07 Published:2014-01-10 Online:2014-01-10

摘要: 在pH=3.22的NaAc-HAc溶液中,应用循环伏安法、方波伏安法、荧光光谱法、紫外可见光谱法和黏度法研究了黄芩素(BAI)与鲱鱼精子DNA(fsDNA)之间的相互作用,发现二者通过沟槽作用形成一电活性较高的复合物,fsDNA为BAI提供了一个低极性的疏水环境导致BAI的峰电流显著增强,增强的峰电流与fsDNA在7.0×10-8~7.0×10-6 g/mL浓度范围内呈正比,由此建立了一种测定核酸的新方法,检测限达到4.1×10-8 g/mL。

关键词: 黄芩素, 核酸, 电化学, 沟槽作用

Abstract: The interaction between fish sperm DNA(fsDNA) and baicalein(BAI) in NaAc-HAc buffer(pH=3.22) was investigated by using cyclic voltammetry, square wave voltammetry, fluorescence spectroscopy, ultraviolet visible spectroscopy and viscosity measurement. Their interaction is in a groove binding mode and the complex has a higher electrical activity. The peak current of BAI is enhanced significantly because fsDNA provides a low polar hydrophobic environment. The enhanced peak current is in proportional to the concentration of fsDNA in the range of 7.0×10-8~7.0×10-6 g/mL. Based on this observstion, a new method with a detection limits at 4.1×10-8 g/mL for the determination of nucleic acids is established.

Key words: baicalein, DNA, electrochemistry, groove binding mode

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