Abstract:

Pseudomonas stutzeri cultured in the lab were chosen to study their influences, including concentration of bacterial solution and bacterial bodies′ solution, on calcium carbonate crystal growth. The kinetics and morphological variation of calcium carbonate were studied by measuring the pH and conductivity of the growth solutions. The products were characterized by XRD, SEM and FT-IR, respectively. Experimental results show that bacterial solution and bacterial bodies′ solution could restrain the calcium carbonate crystallization process. Such effect could be enhanced with the increase of the concentration of bacterial solution or bacterial bodies′ solution. On the basis of these results, it is fairly to speculate that the electrostatic and coordination interactions between polar groups of organic substances and Ca²⁺ are responsible for this morphological change. The bacterial solution could induce the formation of spherical metastable vaterite. The bacterial bodies′ solution could induce the formation of hollow calcite.

Key words: pseudomonas, calciumcarbonate, mineralization, morphology