Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage

doi:10.3724/SP.J.1095.2014.30179

Chinese Journal of Applied Chemistry

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Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage

AN Lingling¹, LU Maofeng¹, DENG Lingling¹, DU Jiangyan^1,2,3*

(1.College of Chemistry and Materials Science,Nanjing Normal University;
2.Jiangsu Key Laboratory of Bio-functional Material;
3.Jiangsu Key Laboratory of New Power Batteries,Nanjing 210046,China)

Received:2013-04-12 Revised:2013-06-16 Published:2014-02-10 Online:2014-02-10

Abstract

Abstract: The electrochemical behavior of 8-hydroxydeoxyguanosine(8-OHdG) at the chitosan(Chi)/graphene(GR) modified glassy carbon electrode(GCE) was investigated by cyclic voltammetry, linear sweep voltammetry and differential pulse voltammetry. A linear relationship between the oxidative peak current and concentrations of 8-OHdG at the modified electrode was established in the range of 3.5×10-7~1.4×10-4 mol/L with a detection limit of 6.4×10-8 mol/L(S/N=3). Chi/GR/GCE was used for the detection of DNA oxidative damage, it showed that the oxidative peak current of 8-OHdG at the modified electrode was linear with concentration of damaged DNA in the range of 10~300 mg/L with a detection limit of 0.026 mg/L(S/N=3).

Key words: hydroxydeoxyguanosine, chitosan, graphene, DNA oxidative damage, electrochemical detection

CLC Number:

O657.1

AN Lingling1, LU Maofeng1, DENG Lingling1, DU Jiangyan1,2,3*. Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30179.

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Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage

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