Chinese Journal of Applied Chemistry ›› 1995, Vol. 0 ›› Issue (3): 70-72.

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Spectral Study on Interactions of Tb3+ and La3+ with Bovine Serum Albumin

Feng Zhixiang1, Zhang Shugong1, Liu Qimin1, Ni Jiazuan1, Miao Jian2, You Shun2   

  1. 1. Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022;
    2. Depeartment of Biochemistry, Henan Medical University, Zhengzhou
  • Received:1994-08-30 Revised:1994-12-31 Published:1995-06-10 Online:1995-06-10

Abstract: The interactions of lanthanium(Ⅲ)and terbium(Ⅲ)ions with bovine serum albu-mine(BSA)in an aqueous solution of hexamethylene-tetramine-HCl buffer and 0.1mol/LNaCl(pH=6.3)have been studied by UV-Vis,IR,CD and fluorescence spectroscopies.Three strong and more than five weak binding sites were found in BSA complex.The sec-ondary structure of BSA was slightly disturbedby coordination with rare earth ions.Groupsin BSA molecule coordinated with rare earth ions are the β-,γ-carboxyls from the side chainsof Asp and Glu residues and the carbonyls from the peptide bonds of BSA.

Key words: lanthanium ion, terbium ion, bovine serum albumin, spectroscopy