Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA

doi:10.3724/SP.J.1095.2014.30232

Chinese Journal of Applied Chemistry

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Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA

GUO Meng^1,2*, WANG Shumin², WANG Jiali², LI Dan², GAO Qingyu^1*, LI Limin²

(1.School of Chemical Engineering and Technology,China University of Mining and Technology,Xuzhou 221116,China;
2.Chemistry & Chemical and Environmental Engineering College,Weifang University,Weifang 261061,China)

Received:2013-05-13 Revised:2013-06-07 Published:2014-01-10 Online:2014-01-10

Abstract

Abstract: The interaction between fish sperm DNA(fsDNA) and baicalein(BAI) in NaAc-HAc buffer(pH=3.22) was investigated by using cyclic voltammetry, square wave voltammetry, fluorescence spectroscopy, ultraviolet visible spectroscopy and viscosity measurement. Their interaction is in a groove binding mode and the complex has a higher electrical activity. The peak current of BAI is enhanced significantly because fsDNA provides a low polar hydrophobic environment. The enhanced peak current is in proportional to the concentration of fsDNA in the range of 7.0×10-8~7.0×10-6 g/mL. Based on this observstion, a new method with a detection limits at 4.1×10-8 g/mL for the determination of nucleic acids is established.

Key words: baicalein, DNA, electrochemistry, groove binding mode

CLC Number:

O657.1

GUO Meng1,2*, WANG Shumin2, WANG Jiali2, LI Dan2, GAO Qingyu1*, LI Limin2. Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA[J]. Chinese Journal of Applied Chemistry, DOI: 10.3724/SP.J.1095.2014.30232.

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Spectroscopic and Electrochemical Methods on the Interaction Mechanism of Baicalein with DNA

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