Chinese Journal of Applied Chemistry

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Preparation of 1, 2, 3-Triazole-Functionalized Polystyrene Diblock Copolymer and Its Properties of Selective Adsorption of Proteins

ZHOU Junhui, WU Qi, LIU Hewen*   

  1. (CAS Key Laboratory of Soft Matter Chemistry,Department of Polymer Science and
    Engineering,University of Science and Technology of China,Hefei 230026,China)
  • Received:2011-05-12 Revised:2011-11-17 Published:2012-05-10 Online:2012-05-10

Abstract: (1-(4-Vinylbenzyl)-1H-1,2,3-triazol-4-yl)methanol(VBTM) monomer was synthesized via click chemistry and RAFT polymerization method was utilized to achieve 1,2,3-triazole-functionalized diblock copolymer(PS-b-PVBTM). The diblock copolymer was spin-coated on gold substrate to form a thin film consisted of nano-spherical structures with enriched PS as core and PVBTM as corona. After being annealed at 90 ℃ for 24 h, the spherical structure was changed to donut-like nano-structure with PS enriched on the surface. Adsorption of proteins on the spin-coated thin film was studied by surface plasma reasonance. The film could absorb bovine serum albumin(BSA) in an acid buffer solution and the absorption capacity was increased as pH decreased while it did not adsorb BSA and myoglobin in a neutral buffer solution. The film adsorbed lysozyme with a pI of 11.0, and the adsorption capacity was weakened by 0.26 ng/mm2 after being annealed. The electrostatic interaction was supposed to be the governing role of adsorption.

Key words: Protein adsorption, diblock copolymers, morphology

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