Preparation and Properties of Epidermal Growth Factor/Shangkehuangshui/Polyvinyl Alcohol Hydrogel Wound Dressing

doi:10.19894/j.issn.1000-0518.250251

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (12): 1649-1660.DOI: 10.19894/j.issn.1000-0518.250251

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Preparation and Properties of Epidermal Growth Factor/Shangkehuangshui/Polyvinyl Alcohol Hydrogel Wound Dressing

Yong-Feng CHANG¹^,², Ming-Feng HE³, Kai-Jun LEI³, Fang-Hao ZHENG³, Xiao-Song ZHOU¹^,², Zhen-Xu WU²(), Zi-Xue JIAO²(), Pei-Biao ZHANG²()

^1.School of Applied Chemistry and Engineering，University of Science and Technology of China，Hefei 230026，China
^2.Key Laboratory of Polymer Ecomaterials，Changchun Institute of Applied Chemistry，Chinese Academy of Sciences，Changchun 130022，China
^3.Foshan Hospital of Traditional Chinese Medicine，Foshan 528000，China

Received:2025-06-20 Accepted:2025-09-11 Published:2025-12-01 Online:2025-12-30
Contact: Zhen-Xu WU,Zi-Xue JIAO,Pei-Biao ZHANG
Supported by:
the Special Fund Project for High-tech Industrialization of Scientific and Technological Cooperation between Jilin Province and the Chinese Academy of Sciences(2022SYHZ0022)

Abstract

Abstract:

Shangkehuangshui （HS） is an in-house preparation of Foshan Traditional Chinese Medicine Hospital， used for the treatment of bone and soft tissue injuries. It exhibits anti-inflammatory， hemostatic， blood circulation-promoting， blood stasis-removing， and new tissue growth-enhancing effects. Currently， in clinical practice， HS is predominantly administered via the “wet compress” method， which has several limitations， including short duration of efficacy， low drug utilization rates， and operational inconvenience. In this study， a hydrogel containing HS was prepared using polyvinyl alcohol （PVA） as a medical excipient through irradiation. The hydrogel tensile strength reaches up to 70 kPa， and elongation at break surpasses 350%. Furthermore， in vitro drug release experiments revealed that both alkaloid and non-alkaloid components of HS achieve effective release. Cell proliferation assays indicated that the number of NIH3T3 cells cultured in PVA/EGF and PVA/HS hydrogels increased significantly by approximately 20% compared to the PVA group within one day following the addition of human epidermal growth factor （EGF）. After seven days， the increase exceeded 30%， with statistically significant results （p＜0.0001）. Rat skin repair experiments demonstrated that HS/PVA hydrogel exhibited comparable efficacy in promoting skin damage repair to the wet compress method of Traumatology Yellow Water Preparation. Moreover， EGF/HS/PVA hydrogel containing EGF showed superior capability in enhancing skin tissue repair. In conclusion， the application of EGF/HS/PVA hydrogel products prepared via irradiation can achieve or even surpass the therapeutic effects of Shangkehuangshui wet application.

Key words: Polyvinyl alcohol, Shangkehuangshui, Epidermal growth factor, Wound dressing

CLC Number:

O632

Yong-Feng CHANG, Ming-Feng HE, Kai-Jun LEI, Fang-Hao ZHENG, Xiao-Song ZHOU, Zhen-Xu WU, Zi-Xue JIAO, Pei-Biao ZHANG. Preparation and Properties of Epidermal Growth Factor/Shangkehuangshui/Polyvinyl Alcohol Hydrogel Wound Dressing[J]. Chinese Journal of Applied Chemistry, 2025, 42(12): 1649-1660.

Figures/Tables 12

References 25

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	MOD	PVA	PVA/EGF1	PVA/EGF10	PVA/EGF100	HP/EGF	HP/EGF1	HP/EGF10	HP/EGF100
10^-9·L·WBC	12.8	6.6	16.1	13.3	6.3	7.7	7.4	8.3	10.4
10^-9·L·LYM	11.1	4.8	13.0	9.6	5.0	6.4	6.0	6.2	8.5
10^-9·L·MO	0.3	0.2	0.3	0.5	0.2	0.1	0.1	0.3	0.2
10^-9·L·GRAN	1.4	1.6	2.8	3.2	1.1	1.2	1.3	1.8	1.7
LYM/%	86.6	73.0	80.5	72.4	79.1	82.4	80.1	74.3	81.4
MO/%	2.7	3.2	2.1	3.9	2.8	1.9	2.0	3.5	2.0
GRAN/%	10.7	23.8	17.4	23.7	18.1	15.7	17.9	22.2	16.6
10^-12·L·RBC	5.66	6.61	4.98	4.13	4.99	6.27	5.52	6.09	5.81
HGB/（g?L^-1）	124	146	139	89	104	129	120	121	119
HCT/%	32.9	38.5	30.6	26.0	27.9	34.6	32.5	32.3	32.7
MCV/fL	58.3	58.3	61.6	63.1	56.0	55.3	59.0	53.1	56.4
MCH/pg	21.9	22.0	27.9	21.5	20.8	20.5	21.7	19.8	20.4
MCHC/（g?L^-1）	376	379	454	342	372	372	369	374	363
RDW/%	12.1	12.5	16.1	14.3	12.4	12.6	12.2	12.5	11.7
10^-9·L·PLT	1184	439	740	906	534	791	584	823	694
MPV/fL	6.2	7.4	6.3	7.8	7.8	6.9	6.7	7.0	6.2
PDW	16.6	17.3	17.3	17.4	17.6	16.6	17.0	17.0	16.6
PCT/%	0.32	0.324	0.001	0.001	0.416	0.545	0.391	0.576	0.430

	MOD	PVA	PVA/EGF1	PVA/EGF10	PVA/EGF100	HP/EGF	HP/EGF1	HP/EGF10	HP/EGF100
10^-9·L·WBC	12.8	6.6	16.1	13.3	6.3	7.7	7.4	8.3	10.4
10^-9·L·LYM	11.1	4.8	13.0	9.6	5.0	6.4	6.0	6.2	8.5
10^-9·L·MO	0.3	0.2	0.3	0.5	0.2	0.1	0.1	0.3	0.2
10^-9·L·GRAN	1.4	1.6	2.8	3.2	1.1	1.2	1.3	1.8	1.7
LYM/%	86.6	73.0	80.5	72.4	79.1	82.4	80.1	74.3	81.4
MO/%	2.7	3.2	2.1	3.9	2.8	1.9	2.0	3.5	2.0
GRAN/%	10.7	23.8	17.4	23.7	18.1	15.7	17.9	22.2	16.6
10^-12·L·RBC	5.66	6.61	4.98	4.13	4.99	6.27	5.52	6.09	5.81
HGB/（g?L^-1）	124	146	139	89	104	129	120	121	119
HCT/%	32.9	38.5	30.6	26.0	27.9	34.6	32.5	32.3	32.7
MCV/fL	58.3	58.3	61.6	63.1	56.0	55.3	59.0	53.1	56.4
MCH/pg	21.9	22.0	27.9	21.5	20.8	20.5	21.7	19.8	20.4
MCHC/（g?L^-1）	376	379	454	342	372	372	369	374	363
RDW/%	12.1	12.5	16.1	14.3	12.4	12.6	12.2	12.5	11.7
10^-9·L·PLT	1184	439	740	906	534	791	584	823	694
MPV/fL	6.2	7.4	6.3	7.8	7.8	6.9	6.7	7.0	6.2
PDW	16.6	17.3	17.3	17.4	17.6	16.6	17.0	17.0	16.6
PCT/%	0.32	0.324	0.001	0.001	0.416	0.545	0.391	0.576	0.430

	MOD	PVA	PVA/EGF1	PVA/EGF10	PVA/EGF100	HP/EGF	HP/EGF1	HP/EGF10	HP/EGF100
UREA/（mol?L^-1）	2.728	7.581	5.774	8.920	6.806	6.491	6.258	6.297	8.126
CREA/（μmol?L^-1）	19.776	48.790	35.739	35.194	48.265	41.917	38.441	41.370	36.679
UA/（μmol?L^-1）	72.830	42.397	105.011	60.451	72.492	51.843	42.836	66.561	64.561
AST/（U?L^-1）	75.756	97.828	72.874	73.987	111.194	99.854	98.395	119.196	70.174
ALT/（U?L^-1）	14.452	22.385	26.184	34.680	39.945	33.075	31.484	35.652	32.171
ALP/（U?L^-1）	45.145	148.724	98.412	149.387	116.321	132.186	137.857	146.472	163.149
TBA/（μmol?L^-1）	24.806	23.440	9.656	14.849	11.812	15.310	33.232	17.144	174.140
ALB/（U?L^-1）	14.946	30.967	30.856	25.255	27.999	29.573	30.700	31.072	27.772
γ-GT/（U?L^-1）	3.153	0.090	0.758	0.881	6.191	1.022	2.889	2.532	5.901
TBIL/（μmol?L^-1）	18.377	9.836	12.194	10.784	9.527	12.395	10.637	7.307	3.299
DBIL/（μmol?L^-1）	11.913	2.313	5.328	1.538	3.033	4.015	2.989	2.575	1.418

	MOD	PVA	PVA/EGF1	PVA/EGF10	PVA/EGF100	HP/EGF	HP/EGF1	HP/EGF10	HP/EGF100
UREA/（mol?L^-1）	2.728	7.581	5.774	8.920	6.806	6.491	6.258	6.297	8.126
CREA/（μmol?L^-1）	19.776	48.790	35.739	35.194	48.265	41.917	38.441	41.370	36.679
UA/（μmol?L^-1）	72.830	42.397	105.011	60.451	72.492	51.843	42.836	66.561	64.561
AST/（U?L^-1）	75.756	97.828	72.874	73.987	111.194	99.854	98.395	119.196	70.174
ALT/（U?L^-1）	14.452	22.385	26.184	34.680	39.945	33.075	31.484	35.652	32.171
ALP/（U?L^-1）	45.145	148.724	98.412	149.387	116.321	132.186	137.857	146.472	163.149
TBA/（μmol?L^-1）	24.806	23.440	9.656	14.849	11.812	15.310	33.232	17.144	174.140
ALB/（U?L^-1）	14.946	30.967	30.856	25.255	27.999	29.573	30.700	31.072	27.772
γ-GT/（U?L^-1）	3.153	0.090	0.758	0.881	6.191	1.022	2.889	2.532	5.901
TBIL/（μmol?L^-1）	18.377	9.836	12.194	10.784	9.527	12.395	10.637	7.307	3.299
DBIL/（μmol?L^-1）	11.913	2.313	5.328	1.538	3.033	4.015	2.989	2.575	1.418

Preparation and Properties of Epidermal Growth Factor/Shangkehuangshui/Polyvinyl Alcohol Hydrogel Wound Dressing

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