低共熔溶剂/表面活性剂体系分离纯化黄芩苷

doi:10.19894/j.issn.1000-0518.210022

摘要/Abstract

摘要： 以低共熔溶剂(DESs)取代传统溶剂,建立了低共熔溶剂-浊点分离(DESs-CPE)体系分离黄芩苷的新方法。研究了包括DESs的类型,DESs中的水含量、料液比、提取温度和时间等因素对黄芩苷提取效率的影响,通过两次浊点分离对黄芩苷进行分离回收以脱除DESs。采用超高效液相色谱方法对黄芩苷进行定量和定性分析。结果表明,DESs中氯化胆碱-丁二酸是最佳提取溶剂,在最优条件下,黄芩苷提取量达(124.73±3.81) mg/g,约为传统方法的1.64倍。两次浊点分离后,黄芩苷最大回收率为(72.46±0.72)%,纯度为(89.72±1.14)%。

关键词: 黄芩苷, 低共熔溶剂, 浊点分离, 回收, 纯化

Abstract: The deep eutectic solvents (DESs) are used to replace the traditional solvents and a new method is established for the separation of baicalin by the eutectic solvent-cloud point separation (DESs-CPE) system. Many variables including the type of DESs in the extractants, the water content in the DES, the solid-liquid ratio, the extraction temperature and time are evaluated and optimized. Two steps of cloud point separations are used to separate and recover baicalin to remove DESs. Quantitative and qualitative analysis of baicalin is carried out by UPLC. Under optimized experimental conditions, the maximum extraction efficiency of baicalin is (124.73±3.81) mg/g, about 1.64 times of the traditional method. After two cloud point separations, the maximum recovery rate of baicalin is (72.46±0.72)% and the purity is (89.72±1.14)%.

Key words: Baicalin, Deep eutectic solvent, Cloud point separation, Recovery, Purification

中图分类号:

O629.9

李叶惠, 李晓宁, 惠伟, 王祥, 王海军. 低共熔溶剂/表面活性剂体系分离纯化黄芩苷[J]. 应用化学, 2021, 38(12): 1647-1654.

LI Ye-Hui, LI Xiao-Ning, HUI Wei, WANG Xiang, WANG Hai-Jun. Separation and Purification of Baicalin Using Deep Eutectic Solvent and Surfactant[J]. Chinese Journal of Applied Chemistry, 2021, 38(12): 1647-1654.

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