中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (01): 83-87.DOI: 10.3724/SP.J.1095.2010.00124

• 研究论文 • 上一篇    下一篇

滇桂艾纳香水溶性多糖BRP-B的分离纯化及止血活性

廖敏富1,林翠梧1*,黄丽1,许子竞1,覃飒飒1,李爱媛2   

  1. (1.广西大学化学化工学院 南宁 530004;2.广西中医学院 南宁)
  • 收稿日期:2010-03-03 修回日期:2010-04-20 出版日期:2011-01-10 发布日期:2011-01-10
  • 通讯作者: 林翠梧，教授; Tel:0771-3275878; E-mail:lincuiwu@163.com; 研究方向：中草药化学成分和天然产物的研究与开发
  • 基金资助:
    国家自然科学基金（20662001,20962002）资助项目,2007年国家大学生创新实验项目（071059314）

Isolation, Purification and Hemostatic Activity of Water-soluble Polysaccharide from Blumea riparia (BI．)DC．

LIAO Min-Fu1, LIN Cui-Wu1*, HUANG Li1, XU Zi-Jing1, QIN Sa-Sa1, LI Ai-Yuan2   

  1. (1.School of Chemistry and Chemical Engineering，Guangxi University，Nanning 530004;
    2.GuangXi Traditional Chinese Medical University，Nanning)
  • Received:2010-03-03 Revised:2010-04-20 Published:2011-01-10 Online:2011-01-10
  • Contact: cuiwu

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987

被引次数

9 | 2

摘要：

用蒸馏水溶解滇桂艾纳香浸膏，从中提取多糖，通过膜分离、三氯乙酸法脱蛋白、二乙氨基乙基(DEAE)脱色、SephadexG10、SephadexG50凝胶柱色谱纯化,获得滇桂艾纳香水溶性多糖(BRP-B)。 由凝胶渗透色谱（HPGPC）确定BRP-B为相对分子质量分布均一的多糖，其数均分子量和质均分子量分别为2654和2716 Da。 以血浆复钙时间（PRT）为指标，研究BRP-B的凝血活性，结果显示BRP-B质量浓度为6.25×10-2 g/L时，凝血时间为248.52 s，凝血时间抑制率达22.42%。 小白鼠离体子宫实验结果显示，BRP-B质量浓度为1.67×10-2、1.97×10-2及2.85×10-2 g/L时能明显增加子宫平滑肌的收缩频率。

关键词: 滇桂艾纳香, 多糖, 分离, 纯化, 止血活性

Abstract:

Water-soluble polysaccharide B(BRP-B) in Blumea riparia(BI.)DC. was extracted and purified by using membrane separation, discoloration on diethylaminoethyl(DEAE)-cellulose, and sephadex column chromatography of G10 and G50. Results from high-performance gel permeation chromatography(HPGPC) showed that the obtained BRP-B has a narrow-distributed molecular mass, with the number average molecular mass and mass average molecular mass as 2654 and 2716, respectively. The drug activity test based on plasma recalcification as the index showed that BRP-B with the optimal concentration of 6.25×10-2 g/L had the strongest procoagulant activity, the coagulation time was 248.52 s, and the inhibition rate of coagulation time was 22.42%. In particularly, BRP-B with concentration of 1.67×10-2, 1.97×10-2 and 2.85×10-2 g/L could significantly enhance the frequency of uterus muscle.

Key words: Blumea riparia (BI．)DC, polysaccharide, separation, purification, hemostatic activity

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