基于多肽-金纳米粒子复合物的生物传感研究及应用进展

doi:10.11944/j.issn.1000-0518.2019.05.180253

摘要/Abstract

摘要：

多肽是α-氨基酸以肽键连接在一起而形成的化合物,同时也是蛋白质水解的中间产物。金纳米粒子具有良好的生物相容性和突出的表面等离子共振性质,基于多肽-金纳米粒子体系的独特光学特性的生物传感研究及应用已引起众多科学工作者的关注。本文仅就近年来利用多肽-金纳米粒子复合物进行生物传感研究及应用加以评述,包括多肽和金纳米粒子的制备与特性、多肽-金纳米粒子复合物的组装以及其在生物传感中的应用等。

关键词: 多肽, 金纳米粒子的修饰, 生物传感

Abstract:

As the intermediate product of protein hydrolysis, peptides are synthesized through the polymerization of α-amino acids. Given the great biocompatibility and surface plasmon resonance(SPR) of gold nanoparticles(AuNPs), biosensors based on the peptide-AuNPs system have attracted the attention of many scientists. In this review, the biosensing research and application of the peptide-AuNPs complexes are summarized, including the preparation and properties of peptides and AuNPs, the synthesis of peptide-AuNPs and the application in biosensing.

Key words: peptide, the modification of gold nanoparticles, biosensing

李昕翼,王小华,周晓东,胡继明. 基于多肽-金纳米粒子复合物的生物传感研究及应用进展[J]. 应用化学, 2019, 36(5): 489-499.

LI Xinyi,WANG Xiaohua,ZHOU Xiaodong,HU Jiming. Research Progress on Biosensing Based on Peptides and Gold Nanoparticles Composite Materials[J]. Chinese Journal of Applied Chemistry, 2019, 36(5): 489-499.

参考文献

[1]	Kim Y,Johnson R C,Hupp J T.Gold Nanoparticle-Based Sensing of “Spectroscopically Silent” Heavy Metal Ions[J]. Nano Lett,2001,1(4):165-167.
[2]	Liu J,Lu Y.A Colorimetric Lead Biosensor Using DNA Zyme-Directed Assembly of Gold Nanoparticles[J]. J Am Chem Soc,2003,125(22):6642-6643.
[3]	Rex M,Hernandez F E,Campiglia A D.Pushing the Limits of Mercury Sensors with Gold Nanorods[J]. Anal Chem,2006,78(2):445-451.
[4]	Lin S Y,Wu S H,Chen C H.A Simple Strategy for Prompt Visual Sensing by Gold Nanoparticles:General Applications of Interparticle Hydrogen Bonds[J]. Angew Chem Int Ed,2006,45(30):4948-4951.
[5]	Zhao W,Chiuman W,Brook M A,et al. Simple and Rapid Colorimetric Biosensors Based on DNA Aptamer and Noncrosslinking Gold Nanoparticle Aggregation[J]. ChemBioChem,2007,8(7):727-731.
[6]	Lytton-Jean A K R,Han M S,Mirkin C A. Microarray Detection of Duplex and Triplex DNA Binders with DNA-Modified Gold Nanoparticles[J]. Anal Chem,2007,79(15):6037-6041.
[7]	LI Jingyun,WANG Yi,XU Jiali,et al. Detection of Cefazolin Based on the Plasmonic Resonance Absorption of Gold Nanoparticle[J]. Chinese J Appl Chem,2012,29(4):455-461(in Chinese). 李靖云,王燚,许佳丽,等. 金纳米颗粒等离子体共振吸收光谱检测头孢唑啉[J]. 应用化学,2012,29(4):455-461.
[8]	Lee J S,Han M S,Mirkin C A.Colorimetrie Detection of Mercuric Ion(Hg2+) in Aqueous Media Using DNA Functionalized Gold Nanoparticles[J]. Angew Chem Int Ed,2007,46(22):4093-4096.
[9]	Medley C D,Smith J E,Tang Z,et al. Gold Nanoparticle-Based Colorimetric Assay for the Direct Detection of Cancerous Cells[J]. Anal Chem,2006,80(4):1067-1072.
[10]	Liu Q,Wang J,Boyd B J.Peptide-Based Biosensors[J]. Talanta,2015,136:114-127.
[11]	Katsoyannis P G.Peptide Synthesis and Protein Structure[J]. J Polym Sci,1961,49(151):51-74.
[12]	HUANG Zhongxian.New Trends in Metalloprotein Studies[J]. Prog Chem,2002,14(4):318-322(in Chinese). 黄仲贤. 金属蛋白研究中几个值得注意的动向[J]. 化学进展,2002,14(4):318-322.
[13]	LI Yuexi,HUANG Peitang.Application of Peptides in Biological Medicine and Diagnosis Reagents[J]. Chinese J Biochem Pharmaceut,2001,22(4):208-210(in Chinese). 李越希,黄培堂. 多肽在生物医药和诊断试剂中的应用[J]. 中华生化药物杂志,2001,22(4):208-210.
[14]	Fujisawa N,Sakao Y,Hayashi S,et al. Alpha-Chemokine Growth Factors for Adenocarcinomas:A Synthetic Peptide Inhibitor for Alpha-Chemokines Inhibits the Growth of Adenocarcinoma Cell Lines[J]. J Cancer Res Clin Oncol,2000,1(1):19-26.
[15]	Llinas-Brunet M,Bailey M,Fazal G.Peptide-Based Inhibitors of the Hepatitis C Virus Serine Protease[J]. Bioorg Med Chem Lett,1998,8(13):1713-1718.
[16]	ZHOU Binbin.Design and Synthesis of Functional Peptides and Their Applications[D]. Hunan:Central South University,2013(in Chinese). 周彬彬. 功能性多肽的设计,合成及其应用研究[D]. 湖南:中南大学,2013.
[17]	Merrifield R B.Solid Phase Peptide Synthesis[J]. J Am Chem Soc,1963,85(14):2149-2154.
[18]	Mitchell A R.Bruce Merrifield and Solid-Phase Peptide Synthesis:A Historical Assessment[J]. Pept Sci,2008,90(3):175-184.
[19]	Alayoglu S,Eichhorn B.Rh-Pt Bimetallic Catalysts:Synthesis, Characterization, and Catalysis of Core-Shell, Alloy, and Monometallic Nanoparticles[J]. J Am Chem Soc,2008,130(51):17479-17486.
[20]	Yu H,Chen M,Rice P M,et al. Dumbbell-Like Bifunctional Au-Fe3O4 Nanoparticles[J]. Nano Lett,2005,5(2):379-382.
[21]	Dijk M A,Tchebotareva A L,Orrit M,et al. Absorption and Scattering Microscopy of Single Metal Nanoparticles[J]. Phys Chem Chem Phys,2006,8(30):3486-3495.
[22]	Wang W,Asher S A.Photochemical Incorporation of Silver Quantum Dots in Monodisperse Silica Colloids for Photonic Crystal Applications[J]. J Am Chem Soc,2001,123(50):12528-12535.
[23]	Li H,Huang H,Feng J,et al. A Polypeptide-Mediated Synthesis of Green Fluorescent Gold Nanoclusters for Fe3+ Sensing and Bioimaging[J]. J Colloid Interface Sci,2017,506:386-392.
[24]	Song S,Qin Y,He Y,et al. Functional Nanoprobes for Ultrasensitive Detection of Biomolecules[J]. Chem Soc Rev,2010,39(11):4234-4243.
[25]	Levy R,Thanh N T K,Doty R C,et al. Rational and Combinatorial Design of Peptide Capping Ligands for Gold Nanoparticles[J]. J Am Chem Soc,2004,126:10076-10084.
[26]	Yan X,Zhu P,Li J.Self-assembly and Application of Diphenylalanine-Based Nanostructures[J]. Chem Soc Rev,2010,6(39):1877-1890.
[27]	Semino C E.Self-assembling Peptides:From Bio-Inspired Materials to Bone Regeneration[J]. J Dent Res,2008,87(7):606-616.
[28]	Zhang S,Holmes T,Lockshin C,et al. Spontaneous Assembly of a Self-complementary Oligopeptide to Form a Stable Macroscopic Membrane[J]. Proc Natl Acad Sci,1993,90(8):3334-3338.
[29]	Zhang S G.Emerging Biological Materials Through Molecular Self-sssembly[J]. Biotechnol Adv,2002,20(5/6):321-339.
[30]	Zhang S G,Yan L,Altman M,et al. Biological Surface Engineering:A Simple System for Cell Pattern Formation[J]. Biomaterials,1999,20(13):1213-1220.
[31]	Gupta M,Bagaria A,Mishra A,et al. Self-assembly of a Dipeptide-Containing Conformationally Restricted Dehydrophenylalanine Residue to Form Ordered Nanotubes[J]. Adv Mater,2007,19(6):858-861.
[32]	Zhang S,Rich A.Direct Conversion of an Oligopeptide from a Beta-Sheet to an Alpha-Helix:A Model for Amyloid Formation[J]. Proc Natl Acad Sci,1997,94(1):23-28.
[33]	Holmes T C.Novel Peptide-Based Biomaterial Scaffolds for Tissue Engineering[J]. Trends Biotechnol,2002,20(1):16-21.
[34]	Ghosh S K,Pal T.Interparticle Coupling Effect on the Surface Plasmon Resonance of Gold Nanoparticles:From Theory to Applications[J]. Chem Rev,2007,107(11):4797-4862.
[35]	Rosi N L,Mirkin C A.Nanostructures in Biodiagnostics[J]. Chem Rev,2005,105(4):1547-1562.
[36]	Niemeyer C M. Nanoparticles, Proteins, Nucleic Acids:Biotechnology Meets Materials Science[J]. Angew Chem Int Ed,2001,40(22):4128-4158.
[37]	Xue W,Zhang G,Zhang D.A Sensitive Colorimetric Label-Free Assay for Trypsin and Inhibitor Screening with Gold Nanoparticles[J]. Analyst,2011,136(15):3136.
[38]	Adj Mian J,Anne A S,Cauet G,et al. Cleavage-Sensing Redox Peptide Monolayers for the Rapid Measurement of the Proteolytic Activity of Trypsin and α-Thrombin Enzymes[J]. Langmuir,2010,26(12):10347-10356.
[39]	Fan H,Jiang X,Zhang T,et al. Peptide-induced Fluorescence Quenching of Conjugated Polyelectrolyte for Label-Free, Ultrasensitive and Selective Assay of Protease Activity[J]. Biosens Bioelectron,2012,34(1):221-226.
[40]	Talley C E,Jusinski L,Hollars C W,et al. Intracellular pH sensors Based on Surface-Enhanced Raman Scattering[J]. Anal Chem,2004,76(23):7064-7068.
[41]	SHU Guowei,CHEN He,LV Jiali.Research Advance of Metal Nanoparticles Prepared by Microorganisms[J]. Funct Mater,2007,38:2052-2055(in Chinese). 舒国伟,陈合,吕嘉枥. 微生物合成金属纳米粒子研究进展[J]. 功能材料,2007,38:2052-2055.
[42]	Turkevich J,Stevenson P C,Hillier J.A Study of the Nucleation and Growth Processes in the Synthesis of Colloidal Gold[J]. Discuss Faraday Soc,1951,11:55.
[43]	Brust M,Walker M,Bethell D,et al. Synthesis of Thiol-Derivatized Gold Nanoparticles in a Two-Phase Liquid-Liquid System[J]. Chem Commun,1994,(7):801-802.
[44]	Farren-Dai M,Awoonor-Williams E,Macneil C S,et al. A Novel Gold Nanoparticle Stabilization and Its Muon Chemistry[J]. Chem Phys Lett,2014,610/611:331-334.
[45]	Zhao P,Li N,Astruc D.State of the Art in Gold Nanoparticle Synthesis[J]. Coord Chem Rev,2013,257(3/4):638-665.
[46]	Daniel M C,Astruc D.Gold Nanoparticles:Assembly, Supramolecular Chemistry, Quantum-Size-Related Properties, and Applications Toward Biology, Catalysis, and Nanotechnology[J]. Chem Rev,2004,104(1):293-346.
[47]	Gupta R K,Srinivasan M P,Dharmarajan R.Synthesis of 16-Mercaptohexadecanoic Acid Capped Gold Nanoparticles and Their Immobilization on a Substrate[J]. Mater Lett,2012,67(1):315-319.
[48]	Boisselier E,Diallo A K,Salmon L,et al. Encapsulation and Stabilization of Gold Nanoparticles with “Click” Polyethyleneglycol Dendrimers[J]. J Am Chem Soc,2010,132(8):2729-2742.
[49]	Pan Y,Guo M,Nie Z,et al. Colorimetric Detection of Apoptosis Based on Caspase-3 Activity Assay Using Unmodified Gold Nanoparticles[J]. Chem Commun,2012,48(7):997-999.
[50]	Bishop K J M,Wilmer C E,Soh S,et al. Nanoscale Forces and Their Uses in Self-assembly[J]. Small,2009,5(14):1600-1630.
[51]	Gill R,Goeken K,Subramaniam V. Fast, Single-Step,Surfactant-Free Oligonucleotide Modification of Gold Nanoparticles Using DNA with a Positively Charged Tail[J]. Chem Commun,2013,49(97):11400-11402.
[52]	Dong Z M,Jin X,Zhao G C.Amplified QCM Biosensor for Type IV Collagenase Based on Collagenase Cleavage of Gold Nanoparticles Functionalized Peptide[J]. Biosens Bioelectron,2018,106:111-116.
[53]	Pan Y L,Guo M L,Nie Z,et al. Colorimetric Detection of Apoptosis Based on Caspase-3 Activity Assay Using Unmodified Gold Nanoparticles[J]. Chem Commun,2012,48(7):997-999.
[54]	Wu Z T,Liu Y Z,Zhou X D,et al. A “Turn-Off” SERS-Based Detection Platform for Ultrasensitive Detection of Thrombin Based on Enzymatic Assays[J]. Biosens Bioelectron,2013,44:10-15.
[55]	Wang X,Geng J,Miyoshi D,et al. A Rapid and Sensitive “Add-Mix-Measure” Assay for Multiple Proteinases Based on One Gold Nanoparticle-Peptide-Fluorophore Conjugate[J]. Biosens Bioelectron,2010,26:743-747.
[56]	Guarise C,Pasquato L,De Filippis V,et al. Gold Nanoparticles-Based Protease Assay[J]. Proc Natl Acad Sci,2006,103(11):3978-3982.
[57]	Wu Z T,Liu Y F,Liu Y Z,et al. A Simple and Universal “Turn-On” Detection Platform for Proteases Based on Surface Enhanced Raman Scattering(SERS)[J]. Biosens Bioelectron,2015,65:375-381.
[58]	Qu G,Zhang G,Wu Z,et al. A “Turn-Off” SERS Assay of Heparin with High Selectivity Based on Heparin-Peptide Complex and Raman Labelled Gold Nanoparticles[J]. Biosens Bioelectron,2014,60:124-129.
[59]	Wu J,Lu Y,Wu Z,et al. Two-Dimensional Molybdenum Disulfide(MoS2) with Gold Nanoparticles for Biosensing of Explosives by Optical Spectroscopy[J]. Sens Actuators B,2018,261:279-287.
[60]	Wang G,Su X,Xu Q,et al. Antifouling Aptasensor for the Detection of Adenosine Triphosphate in Biological Media Based on Mixed Self-assembled Aptamer and Zwitterionic Peptide[J]. Biosens Bioelectron,2018,101:129-134.
[61]	Slocik J M,Zabinski J S,Phillips D M,et al. Colorimetric Response of Peptide-Functionalized Gold Nanoparticles to Metal Ions[J]. Small,2008,4(5):548-551.
[62]	Chai F,Wang C,Wang T,et al. Colorimetric Detection of Pb2+ Using Glutathione Functionalized Gold Nanoparticles[J]. ACS Appl Mater Interfaces,2010,2(5):1466-1470.
[63]	Parnsubsakul A,Oaew S,Surareungchai W.Zwitterionic Peptide-Capped Gold Nanoparticles for Colorimetric Detection of Ni2+[J]. Nanoscale,2018,10(12):5466-5473.
[64]	Aili D,Enander K,Rydberg J,et al. Folding Induced Assembly of Polypeptide Decorated Gold Nanoparticles[J]. J Am Chem Soc,2008,130(17):5780-5788.
[65]	Li X,Wu Z,Zhou X,et al. Colorimetric Response of Peptide Modified Gold Nanoparticles:An Original Assay for Ultrasensitive Silver Detection[J]. Biosens Bioelectron,2017,92:496-501.
[66]	ZHU Zhijun,LIU Dianjun,WANG Zhenxin.A Simple Colorimetric Assay for Detection of Cadmium Ion Based on 6-Mercaptopurine/Peptide Functionalized Gold Nanoparticles[J]. Chinese J Appl Chem,2013,30(6):716-721(in Chinese). 朱志军,刘殿骏,王振新. 基于巯基嘌呤/多肽修饰的金纳米粒子的比色方法检测溶液中的镉离子[J]. 应用化学,2013,30(6):716-721.
[67]	Zhou G,Liu Y,Luo M,et al. Peptide-Capped Gold Nanoparticle for Colorimetric Immunoassay of Conjugated Abscisic Acid[J]. ACS Appl Mater Interfaces,2012,4(9):5010-5015.
[68]	Cortez J,Vorobieva E,Gralheira D,et al. Bionanoconjugates of Tyrosinase and Peptide-Derivatized Gold Nanoparticles for Biosensing of Phenolic Compounds[J]. J Nanopart Res,2011,13:1101-1113.
[69]	Hiroshi T,Tomoko S,Hiroyuki O,et al. Gold Nanoparticles Conjugated with Glycopeptides for Lectin Detection and Imaging on Cell Surface[J]. Protein Pept Lett,2018,25(1):84-89.
[70]	Zhang M,Liu Y Q,Ye B C.Colorimetric Assay for Parallel Detection of Cd2+, Ni2+ and Co2+ Using Peptide-Modified Gold Nanoparticles[J]. Analyst,2012,137(3):601-607.
[71]	Liu X,Wang Y,Chen P,et al. Peptide Functionalized Gold Nanoparticles with Optimized Particle Size and Concentration for Colorimetric Assay Development:Detection of Cardiac Troponin I[J]. ACS Sens,2016,1:1416-1422.
[72]	Li X,Wang J,Sun L.Gold Nanoparticle-Based Colorimetric Assay for Selective Detection of Aluminium Cation on Living Cellular Surfaces[J]. Chem Commun,2010,46(6):988-990.
[73]	Huang H,Zhang Q,Luo J,et al. Sensitive Colorimetric Detection of Lysozyme in Human Serum Using Peptide-Capped Gold Nanoparticles[J]. Anal Methods,2012,4(11):3874-3878.
[74]	Li X Y,Feng F Y,Wu Z T,et al. High Stability of Gold Nanoparticles Towards DNA Modification and Efficient Hybridization via a Surfactant-Free Peptide Route[J]. Chem Commun,2017,53(87):11909.