一种基于喹唑啉酮衍生物的荧光探针对次氯酸根离子的识别

doi:10.11944/j.issn.1000-0518.2017.12.170316

摘要/Abstract

摘要：

次氯酸根(ClO^-)在人体免疫系统中发挥着重要的作用,其识别与检测备受关注。本文设计合成了一种含有喹唑啉酮骨架的腙型荧光探针(HEMQ),并通过¹H NMR、¹³C NMR、高分辨质谱(HRMS)表征了其结构。探针HEMQ在V(乙醇):V(水)=1:1(c(PBS)=0.02 mol/L,pH=8.7)溶液中对ClO^-具有良好的选择性且响应快速,荧光发生显著猝灭。探针HEMQ对ClO^-具有较高的灵敏度,检测限为1.0×10^-4 mol/L。此外,ClO^-可引起探针溶液由黄色到无色的颜色变化,因此HEMQ可作为比色、荧光双通道响应的ClO^-探针。

关键词: 喹唑啉酮, 荧光探针, 次氯酸根, 识别

Abstract:

Hypochlorite(ClO^-) displays essential roles in human immune system, the detection of which has aroused extensive attention. Herein, a hydrazone type fluorescent probe(HEMQ) that containing quinazolinone skeleton was designed and synthesized, and the structure of HEMQ was characterized by ¹H NMR, ¹³C NMR, and HRMS analysis. In V(ethanol):V(water)=1:1(c(PBS)=0.02 mol/L, pH=8.7) solution, probe HEMQ exhibits rapid response and good selectivity toward ClO^- through distinct fluorescence quenching. HEMQ displays good sensitivity to ClO^- with a detection limit of 1.0×10^-4 mol/L. In addition, ClO^- can induce a color change from yellow to colorless of the probe solution, thus, probe HEMQ can act as a colorimetric and florescent dual-channel ClO^- probe.

Key words: quinazolinone, fluorescent probe, hypochlorite, recognition

姜亚男, 汤立军, 边延江, 钟克利, 侯淑华. 一种基于喹唑啉酮衍生物的荧光探针对次氯酸根离子的识别[J]. 应用化学, 2017, 34(12): 1462-1467.

JIANG Ya'nan, TANG Lijun, BIAN Yanjiang, ZHONG Keli, HOU Shuhua. A Quinazolinone Derivative-based Fluorescent Probe for Hypochlorite Ion Recognition[J]. Chinese Journal of Applied Chemistry, 2017, 34(12): 1462-1467.

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