中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (01): 101-106.DOI: 10.3724/SP.J.1095.2010.00174

• 研究论文 • 上一篇    下一篇

叶酸的能量转移荧光测定法及其应用

郭蒙1*,杨景和2   

  1. (1.潍坊学院化学化工学院 潍坊 261061；2.山东大学化学与化工学院 济南)
  • 收稿日期:2010-03-26 修回日期:2010-05-15 出版日期:2011-01-10 发布日期:2011-01-10
  • 通讯作者: 郭蒙，工程师; Tel:0536-8785283; E-mail:guomeng7101@126.com; 研究方向：功能配合物、生物分析化学
  • 基金资助:
    国家自然科学基金（20575035）和潍坊市科技发展计划（2060402）资助项目

A Fluorescent Energy Transfer Method and Its Application in the Detection of Folic Acid

GUO Meng1*, YANG Jinghe2   

  1. (1.School of Chemistry and Chemical Engineering,Weifang University,Weifang 261061;2.School of Chemistry and Chemical Engineering,Shandong University,Ji′nan)
  • Received:2010-03-26 Revised:2010-05-15 Published:2011-01-10 Online:2011-01-10

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1614

被引次数

1

摘要：

报道了一种灵敏检测叶酸的荧光新方法。 在叶酸-KMnO4-Eu3+-H2O2体系中，以pH=4.7的乙酸-乙酸钠（浓度为0.2 mol/L）为缓冲溶液，利用高锰酸钾和过氧化氢将叶酸氧化形成蝶呤-6-羧酸，与Eu3+配位成配合物，该配合物被激发后，蝶呤-6-羧酸通过分子内能量转移将吸收的能量传递到Eu3+，从而发射出Eu3+的特征荧光。 在4×10-8~4×10-5 mol/L范围内，Eu3+的荧光强度与叶酸的浓度呈线性关系，相关系数为0.9902，检测限达到1.2×10-8 mol/L。 用于实际样品中叶酸的检测，得到了满意的结果。

关键词: 叶酸, 铕, 荧光

Abstract:

The detection of folic acid by a fluorescent energy transfer method has been developed. In the folic acid-KMnO4-Eu3+-H2O2 system with the presence of HAc-NaAc buffer solution（pH=4.7, 0.2 mol/L), the folic acid can be oxidized by KMnO4 with the presence of H2O2 and converted into the purine of butterfly-6-carboxyl acid, which can coordinate with Eu3+ forming a complex. The experimental conditions including the concentration of buffer solution and pH value, choice of oxidant, the temperature and heating time, the concentration of Eu3+, etc., were optimized. This system can emit the characteristic fluorescence of Eu3+ through an intra-molecule energy transfer process. The fluorescent intensity is in proportional to the concentration of folic acid in the range of 4×10-8~4×10-5 mol/L with correlation coefficient of 0.9902. The detection limit is found to be 1.2×10-8 mol/L. The developed method has been successfully applied to the determination of folic acid in the actual samples.

Key words: folic acid, Eu, fluorescence

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