新型水溶性罗丹明类荧光探针的合成及其对游离3价金属离子的识别

doi:10.19894/j.issn.1000-0518.210041

摘要/Abstract

摘要：

以罗丹明B为原料，设计合成一种新型水溶性荧光探针（L），并对其进行表征和光学性能研究。结果表明，探针L能在纯水体系（TRIS，pH＝7.4）中快速识别游离M³⁺（Cr³⁺、Al³⁺、Fe³⁺）金属离子，为荧光增强型探针，具有灵敏度高、抗干扰能力强的特点。探针L对Cr³⁺、Al³⁺、Fe³⁺的荧光检测限分别为1.57×10^－5、1.43×10^－5和6.55×10^－5mol／L。表明探针L对生命体系或环境中3价金属离子的检测有潜在应用价值。

关键词: 探针, 离子识别, Fe³⁺, Al³⁺, Cr³⁺

Abstract:

A novel water-soluble probe (L) is designed and synthesized based on rhodamine B. The results indicate that probe L is a "turn-on" fluorescent probe with high selectivity for free M³⁺ (Cr³⁺, Al³⁺, Fe³⁺) detection in pure water system (TRIS, pH=7.4). The detection limits of probe L to Cr³⁺, Al³⁺, and Fe³⁺ are 1.57×10^-5, 1.43×10^-5, and 6.55×10^-5mol/L, respectively. The probe L has potential applications in detection of trivalent metal ions in biological systems and environments.

Key words: Probe, Ion recognition, Fe³⁺, Al³⁺, Cr³⁺

中图分类号:

O657

侯淑华, 李仕琦, 汤立军. 新型水溶性罗丹明类荧光探针的合成及其对游离3价金属离子的识别[J]. 应用化学, 2022, 39(02): 241-246.

Shu-Hua HOU, Shi-Qi LI, Li-Jun TANG. Synthesis of a Novel Water-Soluble Rhodamine-Based Fluorescent Probe and Its Selective Detection of Free Trivalent Ions[J]. Chinese Journal of Applied Chemistry, 2022, 39(02): 241-246.

图/表 6

图示1　探针L的合成

Scheme 1　Synthesis of probe L

图1 （A）探针L（1.0×10－5mol／L）中加入各种金属离子（10 mmol／L）的紫外吸收光谱；（B）探针L（1.0×10－5mol／L）中加入各种金属离子（10 mmol／L）的荧光光谱。其中：（a）探针L中加入Fe3+；（b）探针L中加入Al3+；（c）探针L中加入Cr3+

Fig.1 （A）The UV absorption spectra of various metal ions（10 mmol／L）added to probe L（1.0×10－5mol／L）.（B）Fluorescence spectra of various metal ions（10 mmol／L）added to probe L（1.0×10－5mol／L）：（a）probe L add Fe3+；（b）probe L add Al3+；（c）probe L add Cr3+

图2 其它金属离子和Cr3+共存时探针L（1.0×10－5mol／L）在TRIS中荧光发射光谱变化（λex＝510 nm）

Fig.2 The fluorescence emission spectra of the probe L（1.0×10－5mol／L）in TRIS（λex＝510 nm）in the coexistence of other metal ions and Cr3+

图3 探针L（1.0×10－5mol／L）中加入不同浓度Cr3+后的荧光光谱的变化曲线图（λex＝510 nm）

Fig.3 Fluorescence emission spectra of probe L in the presence of Cr3+with different concentrations（λex＝510 nm）

图4 探针L与Cr3+的Job′s plot图

Fig.4 Job′s plot of probe L with Cr3+

图示2　探针L识别机理

Scheme 2　Cooperation mecanism of probe L

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