反式白藜芦醇双功能单体印迹固相萃取柱的制备及应用

doi:10.11944/j.issn.1000-0518.2015.11.150137

摘要/Abstract

摘要：

采用自组装技术,分别以β-环糊精和甲基丙烯酸为功能单体,反式白藜芦醇为模板分子,乙二醇二甲基丙烯酸酯为交联剂,制备了一种双功能单体印迹聚合物。并将其作为固相萃取材料萃取红酒和白酒酒样中的反式白藜芦醇。结果表明,与单功能印迹材料和非印迹材料相比,这种双功能单体印迹材料具有更高的吸附容量和选择性;结合高效液相色谱,建立了酒样中反式白藜芦醇浓度的检测方法。该方法的线性范围在0.003~2 mg/L,红酒的回收率在87.9%~93.2%,白酒的回收率在89.3%~91.2%,检测限为0.001 mg/L。

关键词: 分子印迹, 反式白藜芦醇, β-环糊精, 固相萃取, 酒样, 高效液相色谱

Abstract:

A novel molecularly imprinted polymer(DMIP) for trans-resveratrol was fabricated with bonded β-cyclodextrin(β-CD) and methacrylic acid as double monomers. Molecularly imprinted solid phase extraction column prepared with bonded double monomers has higher imprinting effect to the template molecule. DMIP prepared using bonded β-CD and methacylic acid, either separately or combined have shown various recognition properties. The results of adsorption experiments indicated that the selectivity of DMIP is superior to those obtained with only methacrylic acid(SMIP). Molecularly imprinted solid phase extraction column was used to determine the trans-resveratrol in wine and Chinese liquor samples. The recoveries of 87.9%~93.2%, 87.9%~93.2% and 89.3%~91.2% are obtained for red wine and white wine and Chinese Liquor, respectively. This method is highly sensitive and specific for the analyte, along with a low limit of determination at 0.001 mg/L and a linear range from 0.003 to 2 mg/L.

Key words: molecularly imprinted polymers, trans-resveratrol, β-cyclodextrin, solid-phase extraction, wine sample, high performance liquid chromatography

李媛媛, 赵川德, 张海霞. 反式白藜芦醇双功能单体印迹固相萃取柱的制备及应用[J]. 应用化学, 2015, 32(11): 1283-1289.

LI Yuanyuan, ZHAO Chuande, ZHANG Haixia. Preparation of Molecularly Imprinted Solid Phase Extraction Column Using Double Functional Monomers for the Determination of trans-Resveratrol[J]. Chinese Journal of Applied Chemistry, 2015, 32(11): 1283-1289.

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