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光散射/荧光比率法测定全氟辛烷磺酸

谭克俊*,吴飞,代旭,郑莉   

  1. (发光与实时分析教育部重点实验室， 西南大学化学化工学院 重庆 400715)
  • 收稿日期:2012-03-27 修回日期:2012-05-08 出版日期:2013-02-10 发布日期:2013-02-10
  • 通讯作者: 谭克俊，副教授; Tel/Fax:023-68367257; E-mail:tankj@swu.edu.cn; 研究方向：环境分析化学
  • 基金资助:
    国家自然科学基金(20877063）西南大学大型仪器设备开放基金(201133)资助项目

Determination of Perfluorooctane Sulfonate by Light Scattering-Fluorescence Ratio Method

TAN Kejun*, WU Fei, DAI Xu, ZHENG Li   

  1. (Key Laboratory on Luminescence and Real-Time Analysis,Ministry of Education,College of
    Chemistry and Chemical Engineering,Southwest University,Chongqing 400715,China)
  • Received:2012-03-27 Revised:2012-05-08 Published:2013-02-10 Online:2013-02-10
  • Contact: tan

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被引次数

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摘要： 在pH=1.81的BrittonRobinson（BR）缓冲溶液中，全氟辛烷磺酸（PFOS）与罗丹明B（RhB）通过静电引力和疏水作用使RhB的光散射增强，荧光发生猝灭。 利用在普通荧光光度计上单次扫描获得的340 nm处的光散射强度（I340）与596 nm处的荧光强度（F596）之比，建立了测定PFOS的光散射/荧光比率法，检出限（3σ）为17 nmol/L。 方法用于环境水样中PFOS的测定，RSD≤4.2%。

关键词: 全氟辛烷磺酸, 罗丹明B, 散射/荧光比率法

Abstract: Perfluorooctane sulfonate(PFOS) can quench the fluorescence of rhodamine B(RhB) in pH=1.81 Britton-Robinson(BR) buffer solution, and results in light scattering enhancement by electrostatic forces and hydrophobic interactions. In this work, a light scattering-fluorescence ratio method of PFOS was established with a common spectrofluorometer by measuring the light emission signals through one scanning, out of which a ratio of the intensity of light scattering at 340 nm to that of fluorescence at 596 nm could be obtained. The linear range is from 0.17 to 10.0 μmol/L, and the limit of detection(3σ) is 17 nmol/L. The method has been applied to the determination of PFOS in the environmental samples with RSD≤4.2%.

Key words: PFOS, rhodamine B, light scattering- fluorescence ratio

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