中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (11): 1362-1364.DOI: 10.3724/SP.J.1095.2010.00002

• 研究简报 • 上一篇    

HPLC法同时测定石蒜中加兰他敏和石蒜碱

李霞1,熊海蓉2,蒋利华3,文祝友4,熊远福1*   

  1. (湖南农业大学1.理学院,2.分析测试中心,4.动物科技学院 长沙 410128;
    3.长沙环境保护职业技术学院 长沙)
  • 收稿日期:2010-01-04 修回日期:2010-06-29 出版日期:2010-11-10 发布日期:2010-11-10
  • 通讯作者: 熊远福，男，教授; E-mail:yuanfuxiong888@163.com; 研究方向：天然产物
  • 基金资助:
    湖南农业大学人才基金（04WD12）资助项目

Determination of Galanthamine and Lycorine in Lycoris Herb. by HPLC

LI Xia1, XIONG Hai-Rong2, JIANG Li-Hua3, WEN Zhu-You4, XIONG Yuan-Fu1*   

  1. (1.College of Science;2.Center of Analysis and Testing;4.College of Animal Science and Technology,
    Hunan Agricultural University,Changsha 410128;
    3.Changsha Environmental Protection College,Changsha)
  • Received:2010-01-04 Revised:2010-06-29 Published:2010-11-10 Online:2010-11-10

PDF

1229

被引次数

44 | 3

摘要：

建立了高效液相色谱同时测定石蒜中加兰他敏和石蒜碱含量的方法。 采用phenomenex-C18色谱柱，以乙腈(A)-0.1%TFA水溶液(B)为流动相梯度洗脱，检测波长289 nm。 结果表明，加兰他敏和石蒜碱均在0.5～10 mg/L(r=0.9999)呈现良好的线性关系，最低检测限(S/N=3)分别为0.09和0.15 mg/L，平均加样回收率分别为99.53%和96.82%。 该方法简单、快速、准确，适合于石蒜中加兰他敏和石蒜碱的测定。

关键词: 高效液相色谱, 石蒜, 加兰他敏, 石蒜碱

Abstract:

A high performance liquid chromatographic method was developed for simultaneous determining the contents of galanthamine and lycorine in Lycoris Herb. The chromatographic analysis was carried out on a phenomenex-C18 column. The mobile phase was acetonitrile(A)-0.1%trifluoroacetic(TFA)(B) with gradient elution. The detection wavelength was set at 289 nm. The calibration curves for galanthamine and lycorine showed good linearity in the range of 0.5～10 mg/L(r=0.9999). The detection limits were 0.09 mg/L for galanthamine and 0.15 mg/L for lycorine at the signal-to-noise ratio of 3, and the average recoveries were 99.53% and 96.82%, respectively. The method is simple, rapid, accurate and suitable for the determination of galanthamine and lycorine in Lycoris Herb.

Key words: HPLC, Lycoris Herb, galanthamine, lycorine

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