中华医学超声杂志

应用化学 ›› 2010, Vol. 27 ›› Issue (04): 484-487.DOI: 10.3724/SP.J.1095.2010.90252

• 研究论文 • 上一篇    下一篇

赖氨酸-甲醇-1,2-萘醌-4-磺酸钠体系褪色光度法测定甲醇

李晶,郑立庆,张甜甜,李全民*   

  1. (河南师范大学化学与环境科学学院，河南省污染控制重点实验室 新乡 453007)
  • 收稿日期:2009-04-09 修回日期:2009-08-01 出版日期:2010-04-10 发布日期:2010-04-10
  • 通讯作者: 李全民,男,教授; E-mail:mercury6068@hotmail.com; 研究方向：药物及环境污染物分析
  • 基金资助:
    河南省科技厅自然科学基金（082300420210;082300420220）资助项目

Decolouring Spectrophotometric Determination of Methanol in Lysine-Methanol-Sodium 1,2-Naphthoquinone-4-sulfonate System

LI Jing, ZHENG Li-Qing, ZHANG Tian-Tian, LI Quan-Min*   

  1. (Henan Key Laboratory for Environmental Pollution Control,College of Chemistry
    and Environmental Science,Henan Normal University,Xinxiang 453007)
  • Received:2009-04-09 Revised:2009-08-01 Published:2010-04-10 Online:2010-04-10

PDF

1405

被引次数

13 | 1

摘要：

赖氨酸与1,2-萘醌-4-磺酸钠在碱性条件下发生亲核取代反应，生成红褐色的产物，在该体系中加入甲醇后发生褪色反应，褪色最大波长λmax=559 nm,甲醇质量浓度在0.050～7.61 g/L范围内与吸光度降低程度呈良好线性关系，线性回归方程为A=-0.005 17-0.086 79c(g/L),线性相关系数r=0.999 6，检测限为49.5 mg/L,平均回收率为99.1%～102.6%。 用于甲醇样品测定，结果满意。

关键词: 分光光度法, 甲醇, 赖氨酸, 萘醌磺酸钠

Abstract:

A simple decolouring spectrophotometric method for the determination of methanol was developed in this work. A russety product can be formed by the reaction between Lysine(Lys) and sodium 1,2-naphthoquinone-4-sulfonate(NQS) in an alkaline solution. When methanol was added to the solution, the system involves decolouring process. Beer′s law is held in a range of methanol concentration of 0.050~7.61 g/L at a maximal decolouring wavelength of 559 nm. The equation of linear regression is A=-0.005 17-0.086 79c(g/L), with a linearly correlation coefficient of 0.999 6. The detection limit of methanol is 49.5 mg/L(3s/k) and the recovery rate is in a range of 99.1%~102.6%. Analytical results obtained with this method are satisfactory.

Key words: spectrophotometry, methanol, L-lysine, Sodium naphthoquinonesulfonate

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