中华医学超声杂志

应用化学 ›› 1994, Vol. 0 ›› Issue (6): 77-80.

• 研究论文 • 上一篇    下一篇

模拟谷胱甘肽过氧化物酶活性三肽的合成及表征

朱振齐1, 孙启安1, 丁兰1, 罗贵民1, 李维21, 沈家骢3   

  1. 1. 吉林大学酶工程国家重点实验室 长春 130023;
    2. 吉林大学分子生物学系 长春;
    3. 吉林大学化学系 长春
  • 收稿日期:1994-03-21 修回日期:1994-07-12 出版日期:1994-12-10 发布日期:1994-12-10

Synthesis and Characterizaion of Mimic Tripeptides with GPX Activity

Zhu Zhenqi1, Sun Qi'an1, Ding Lan1, Luo Guimin1, Li Wei21, Shen Jiacong3   

  1. 1. The State Key Laboratory of Enzyme Engineering, Jilin University, Changchun 130023;
    2. Department of Molecnlar Biology, Jilin University, Changchun;
    3. Department of Chemistry, Jilin University, Changchun
  • Received:1994-03-21 Revised:1994-07-12 Published:1994-12-10 Online:1994-12-10

PDF

428

被引次数

4 | 1

摘要： 用B,B-二氟硼杂唑烷酮保护谷氨酸的α-氨基和α-羧基,通过液相合成法制得谷氨酰-γ-丝氨酰-甘氨酸三肽。结合苯甲基磺酰氟(PMSF)与硒化氢(H2Se)的突变,再经纯化制得具有谷胱甘肽过氧化物酶活性的三肽模拟物,即γ-谷氨酰-硒代半腕氨酰-甘氨酸。元素分析、氨基酸分析均证明了两种三肽的结构。电子能谱技术分析测得硒的价态及含量。

关键词: 三肽模拟物, 谷胱甘肽过氧化物酶, 合成, 表征

Abstract: The alpha-amino and alpha-carboxyl group in glutamic acid (Glu) were protected by specific coprotection method,γ-Glu-Ser-Gly was synthesized by the liquid peptide method. The prepared mimic tripeptides were used for the chemical mutation of PMSF and H2Se, as well as γ-Glu-SeCys-Gly, The structures of mimic tripeptides were determined by elemental analysis and amino acid analysis. The valency and quantity of Se in mimic tripeptides were determined.

Key words: mimic tripeptide, GPX, synthesis, characterization

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