中华医学超声杂志

应用化学 ›› 1990, Vol. 0 ›› Issue (2): 71-73.

• 研究简报 • 上一篇    下一篇

铬-硫胺素荧光光度法测定铬

揭念琴, 于文光   

  1. 山东大学化学系 济南 250100
  • 收稿日期:1989-06-22 修回日期:1989-09-04 出版日期:1990-04-10 发布日期:1990-04-10

FLUORIMETRIC DETERMINATION OF CHROMIUM(Ⅵ)THROUGH Cr(Ⅳ)-THIAMINE REACTION

Jie Nianqin, Yu Wenguang   

  1. Department of Chemistry, Shandong University, Jinan 250100
  • Received:1989-06-22 Revised:1989-09-04 Published:1990-04-10 Online:1990-04-10

PDF

371

被引次数

2

摘要： 荧光法测定铬已有报道[1-7],但以氧化还原荧光反应为基础的测定方法尚未见报道。木文研究了铬(Ⅳ)-硫胺素(VB1)体系,发现在3.5—4.0mol/L的硫酸介质中,铬(Ⅵ)-硫胺素反应生成荧光物质硫胺荧:硫胺素+Cr2O72-⇔硫胺荧+2Cr3+。硫胺荧的激发波长和发射波长分别为λex=405mm,λem=465nm。可以用来作为Cr(Ⅵ)的荧光定量分析。一般荧光法测定铬的方法[1,2]虽灵敏度较高,但需有机试剂萃取,手续麻烦。本方法则可直接在水相中进行。

关键词: 铬, 硫胺素, 荧光分析

Abstract: Based on the reaction of chromind(Ⅵ)with thiamine hydrochloride a simple, sensitivemethod,for. the fluorimetric deteamination of chromium is developed. The fluoresent specieshas an excitation and emission maxima at 405nm and 465nm respectively. The sensitivity ofthe determination of chromium is 1×10-6mol/L. The method has been applied to determinechromium in wast water and cast iron satisfactorily.

Key words: chromium, thiochrome, fluorescence analysis

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