中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (09): 1046-1051.DOI: 10.3724/SP.J.1095.2011.00568

• 研究论文 • 上一篇    下一篇

聚多巴胺/多壁碳纳米管/玻璃碳电极上多巴胺的电分析

李云龙,苏招红,陈超,孟越,谢青季*   

  1. (湖南师范大学化学生物学及中药分析省部共建教育部重点实验室 长沙 410081)
  • 收稿日期:2010-09-27 修回日期:2011-01-16 出版日期:2011-09-10 发布日期:2011-09-10
  • 通讯作者: 谢青季，教授; Tel:0731-88865515; Fax:0731-88872046; E-mail:xieqj@hunnu.edu.cn; 研究方向：电分析化学和生物传感器
  • 基金资助:
    国家自然科学基金（21075036, 90713018）和湖南省教育厅科研（10C0927）资助项目

Electroanalysis of Dopamine at Polydopamine/Multiwalled Carbon Nanotubes/Glassy Carbon Electrode

LI Yunlong, SU  Zhaohong, CHEN Chao, MENG Yue, XIE Qingji*   

  1. (Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research
    (Ministry of Education),Hunan Normal University,Changsha 410081)
  • Received:2010-09-27 Revised:2011-01-16 Published:2011-09-10 Online:2011-09-10

PDF

2542

被引次数

10

摘要：

基于多巴胺（DA）在多壁碳纳米管（MWCNTs）修饰玻璃碳（GC）电极上的电聚合，制得聚多巴胺(PDA)/MWCNTs/GC电极，并对该修饰电极进行了电化学阻抗谱 (EIS)和循环伏安法(CV)表征。 在该修饰电极上，DA呈现良好的电化学行为。在pH=7.4磷酸缓冲溶液中其氧化电流显著高于在裸电极上的响应，且能有效地抑制2.0 mmol/L抗坏血酸(AA)或K4Fe(CN)6的直接电化学响应，表明MWCNTs可增敏信号，且阳离子选择透过性PDA膜可抑制阴离子的电化学干扰。 采用CV实验检测DA，DA氧化的半微分伏安峰高(ipa-sd)与多巴胺浓度在0.08～1.76 μmol/L范围内呈线性关系，在无抗坏血酸和有0.5 mmol/L抗坏血酸共存时的线性回归方程分别为ipa-sd(μA/s1/2)=0.107+0.405c(μmol/L)（r2=0.986）和ipa-sd(μA/s1/2)=0.628+0.649c(μmol/L)(r2=0.992),检测限均为8.0×10-8 mol/L(S/N=3)。 该法用于盐酸多巴胺注射液中多巴胺的快速测定，结果满意。

关键词: 聚多巴胺/多壁碳纳米管/玻璃碳电极, 电催化, 干扰抑制, 多巴胺电分析

Abstract:

On the basis of electropolymerization of dopamine(DA) at multiwalled carbon nanotubes(MWCNTs) modified glassy carbon(GC) electrodes, polydopamine(PDA)/MWCNTs/GC electrodes were prepared and characterized by electrochemical impedance spectroscopy(EIS) and cyclic voltammetry(CV). The electrode exhibited well-defined electrochemical behavior of DA in pH=7.4 phosphate buffer, producing higher oxidation currents than those on a bare GC electrode, and the direct electrochemical responses of 2.0 mmol/L ascorbic acid(AA) or K4Fe(CN)6 were inhibited, demonstrating the nano-enhancement effect of MWCNTs and the anti-anion electroanalysis selectivity due to use of the PDA film with the selective cation-permeability nature. DA electroanalysis was conducted at the electrode using CV and semi-derivative methods. The semi-derivative voltammetric peak height for DA oxidation is linear with DA concentration in the range of 0.08～1.76 μmol/L, and the linear regression equation in a phosphate buffer solution without AA or with 0.5 mmol/L coexisting AA is ipa-sd(μA/s1/2)=0.107+0.405c(μmol/L)(r2=0.986) or ipa-sd(μA/s1/2)=0.628+0.649c(μmol/L)(r2=0.992), both with detection limits of 8.0×10-8 mol/L(S/N=3). This method was used for rapid determination of DA in a DA hydrochloride injection sample with satisfactory results.

Key words: PDA/MWCNTs/GC electrode, electroanalysis, interference inhibition, DA electroanalysis.

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