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应用化学 ›› 2024, Vol. 41 ›› Issue (5): 687-702.DOI: 10.19894/j.issn.1000-0518.230318
胡轩1, 吴同川1, 艾鑫丹1, 何兴悦2, 徐弘康1, 郑飞1, 越皓1(), 戴雨霖1(
)
收稿日期:
2023-10-12
接受日期:
2024-03-09
出版日期:
2024-05-01
发布日期:
2024-06-03
通讯作者:
越皓,戴雨霖
基金资助:
Xuan HU1, Tong-Chuan WU1, Xin-Dan AI1, Xing-Yue HE2, Hong-Kang XU1, Fei ZHENG1, Hao YUE1(), Yu-Lin DAI1(
)
Received:
2023-10-12
Accepted:
2024-03-09
Published:
2024-05-01
Online:
2024-06-03
Contact:
Hao YUE,Yu-Lin DAI
About author:
daiyl@ccucm.edu.cnSupported by:
摘要:
采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UHPLC-Q-Exactive Orbitrap MS)技术,结合网络药理学和分子对接,对少腹逐瘀汤发挥抗宫颈癌作用的重要成分、靶点和通路进行预测分析,并通过细胞实验初步探究其作用机制。 经鉴定发现苯丙素类、黄酮类、萜类、生物碱类、有机酸类、挥发油类、苯酞类、香豆素类、苯醛类、氨基酸类、酚类和其他类等共89种化合物,网络药理学和分子对接深入挖掘得到少腹逐瘀汤抗宫颈癌的7个重要成分、8个核心靶点和10条宫颈癌相关信号通路。 细胞实验表明,给药组HeLa细胞与空白对照组相比,生存率逐渐降低,细胞凋亡率逐步提高,呈浓度依赖性(P<0.05)。 少腹逐瘀汤可以提高Bax、Cyto c、Caspase 9和Caspase 3表达水平,降低PI3K、AKT和Bcl-2表达水平。 少腹逐瘀汤可能通过槲皮素、芍药苷、黄芩素、山奈酚、沙立朴吩、丁香酚和鞣花酸等成分作用于TP53、AKT1和EGFR等靶点发挥抗宫颈癌作用,其机制与PI3K/AKT和Bcl-2家族蛋白信号通路的调控密切相关,充分体现了多成分、多靶点和多通路的特点。
中图分类号:
胡轩, 吴同川, 艾鑫丹, 何兴悦, 徐弘康, 郑飞, 越皓, 戴雨霖. 液质联用结合网络药理学探究少腹逐瘀汤抗宫颈癌活性机理[J]. 应用化学, 2024, 41(5): 687-702.
Xuan HU, Tong-Chuan WU, Xin-Dan AI, Xing-Yue HE, Hong-Kang XU, Fei ZHENG, Hao YUE, Yu-Lin DAI. The Anti-Cervical Cancer Mechanism of Shaofu Zhuyu Decoction Based on Liquid Chromatography Mass Spectrometry Combined with Network Pharmacology[J]. Chinese Journal of Applied Chemistry, 2024, 41(5): 687-702.
Mol ID | Compounds | OB/% | DL | Origin |
---|---|---|---|---|
MOL001918 | Paeoniflorgenone | 87.59 | 0.37 | Paeoniae radix rubra |
MOL001925 | Paeoniflorin_qt | 68.18 | 0.40 | Paeoniae radix rubra |
MOL007016 | Paeoniflorigenone | 65.33 | 0.37 | Paeoniae radix rubra |
MOL006996 | 1-o-beta-d-Glucopyranosylpaeonisuffrone_qt | 65.08 | 0.35 | Paeoniae radix rubra |
MOL007022 | EvofolinB | 64.74 | 0.22 | Paeoniae radix rubra |
MOL007018 | 9-Ethyl-neo-paeoniaflorin A_qt | 64.42 | 0.30 | Paeoniae radix rubra |
MOL006992 | (2R,3R)-4-Methoxyl-distylin | 59.98 | 0.30 | Paeoniae radix rubra |
MOL007008 | 4-Ethyl-paeoniflorin_qt | 56.87 | 0.44 | Paeoniae radix rubra |
MOL007012 | 4-o-Methyl-paeoniflorin_qt | 56.7 | 0.43 | Paeoniae radix rubra |
MOL000492 | (+)-Catechin | 54.83 | 0.24 | Paeoniae radix rubra |
MOL000433 | FA | 68.96 | 0.71 | Chuanxiong rhizome |
MOL002140 | Perlolyrine | 65.95 | 0.27 | Chuanxiong rhizome |
MOL002151 | Senkyunone | 47.66 | 0.24 | Chuanxiong rhizome |
MOL002157 | Wallichilide | 42.31 | 0.71 | Chuanxiong rhizome |
MOL001494 | Mandenol | 42.00 | 0.19 | Chuanxiong rhizome |
MOL002135 | Myricanone | 40.60 | 0.51 | Chuanxiong rhizome |
MOL000359 | Sitosterol | 36.91 | 0.75 | Chuanxiong rhizome, Zingiberis rhizoma |
MOL002514 | Sexangularetin | 62.86 | 0.30 | Zingiberis rhizoma |
MOL002501 | [(1S)-3-[(E)-but-2-enyl]-2-methyl-4-oxo-1-cyclopent-2-enyl] (1R,3R)-3-[(E)-3-methoxy-2-methyl-3-oxoprop-1-enyl]-2,2-dimethylcyclopropane-1-carboxylate | 62.52 | 0.31 | Zingiberis rhizoma |
MOL002464 | 1-Monolinolein | 37.18 | 0.30 | Zingiberis rhizoma |
MOL000358 | beta-Sitosterol | 36.91 | 0.75 | Zingiberis rhizoma, Angelica sinensis radix, Foeniculi fructus, Typhae pollen |
MOL000979 | 2-Methoxyfuranoguaia-9-ene-8-one | 66.18 | 0.18 | Myrrha |
MOL001033 | Diayangambin | 63.84 | 0.81 | Myrrha |
MOL001031 | Epimansumbinol | 61.81 | 0.40 | Myrrha |
MOL001131 | Phellamurin_qt | 56.6 | 0.39 | Myrrha |
MOL001009 | Guggulsterol-VI | 54.72 | 0.43 | Myrrha |
MOL000988 | 4,17(20)-(cis)-Pregnadiene-3,16-dione | 51.42 | 0.48 | Myrrha |
MOL001013 | Mansumbinoic acid | 48.10 | 0.32 | Myrrha |
MOL000098 | Quercetin | 46.43 | 0.28 | Myrrha, Typhae pollen |
MOL001028 | (8R)-3-oxo-8-Hydroxy-polypoda -13E,17E,21-triene | 44.83 | 0.59 | Myrrha |
MOL001088 | 1α-Acetoxy-9,19-cyclolanost-24-en-3β-ol | 44.40 | 0.78 | Myrrha |
MOL006115 | Kaempferol-3-O-α-L-rhamnosyl(1→2)-β-D-glucoside_qt | 62.87 | 0.24 | Typhae pollen |
MOL000354 | Isorhamnetin | 49.60 | 0.31 | Typhae pollen |
MOL001439 | Arachidonic acid | 45.57 | 0.20 | Typhae pollen |
MOL001040 | (2R)-5,7-Dihydroxy-2-(4-hydroxyphenyl)chroman-4-one | 42.36 | 0.21 | Typhae pollen |
MOL000422 | Kaempferol | 41.88 | 0.24 | Typhae pollen |
MOL006111 | Testosterone palmitate | 34.14 | 0.71 | Typhae pollen |
MOL000449 | Stigmasterol | 43.83 | 0.76 | Angelica sinensis radix, Foeniculi fructus |
MOL000254 | Eugenol | 56.24 | 0.04 | Cinnamomi cortex |
MOL000118 | L-alpha-terpineol | 48.80 | 0.03 | Cinnamomi cortex |
MOL000198 | (R)-Linalool | 39.80 | 0.02 | Cinnamomi cortex |
MOL000475 | Anethole | 32.49 | 0.03 | Cinnamomi cortex |
MOL000991 | Cinnamaldehyde | 31.99 | 0.02 | Cinnamomi cortex |
MOL002380 | cis-Anethol | 30.86 | 0.03 | Cinnamomi cortex |
MOL000704 | Styrene | 29.55 | 0.01 | Cinnamomi cortex |
MOL000431 | Coumarin | 29.17 | 0.04 | Cinnamomi cortex |
MOL000250 | cis-Cinnamaldehyde | 27.21 | 0.02 | Cinnamomi cortex |
MOL000024 | alpha-Humulene | 22.98 | 0.06 | Cinnamomi cortex |
MOL001941 | Ammidin | 34.55 | 0.22 | Foeniculi fructus |
MOL004193 | Clarkeanidine | 86.65 | 0.54 | Corydalis rhizoma |
MOL001460 | Cryptopin | 78.74 | 0.72 | Corydalis rhizoma |
MOL004234 | 2,3,9,10-Tetramethoxy-13-methyl-5,6-dihydroisoquinolino[2,1-b]isoquinolin-8-one | 76.77 | 0.73 | Corydalis rhizoma |
MOL004071 | Hyndarin | 73.94 | 0.64 | Corydalis rhizoma |
MOL000791 | Bicuculline | 69.67 | 0.88 | Corydalis rhizoma |
MOL004195 | CORYDALINE | 65.84 | 0.68 | Corydalis rhizoma |
MOL000785 | Palmatine | 64.60 | 0.65 | Corydalis rhizoma |
MOL004191 | Capaurine | 62.91 | 0.69 | Corydalis rhizoma |
MOL004200 | Methyl-[2-(3,4,6,7-tetramethoxy-1-phenanthryl)ethyl]amine | 61.15 | 0.44 | Corydalis rhizoma |
MOL001463 | Dihydrosanguinarine | 59.31 | 0.86 | Corydalis rhizoma |
WLZ000001 | Ponalactone A | - | - | Trogopterus dung |
WLZ000002 | Uric acid | - | - | Trogopterus dung |
WLZ000003 | Wulingzhic acid | - | - | Trogopterus dung |
WLZ000004 | Japondipsaponin E1 | - | - | Trogopterus dung |
WLZ000005 | Urea | - | - | Trogopterus dung |
WLZ000006 | Trihydroxy isosterocholenic acid | - | - | Trogopterus dung |
表1 少腹逐瘀汤的候选活性成分 (Continued from previous page)
Table 1 Candidate active ingredients of Shaofu Zhuyu Decoction
Mol ID | Compounds | OB/% | DL | Origin |
---|---|---|---|---|
MOL001918 | Paeoniflorgenone | 87.59 | 0.37 | Paeoniae radix rubra |
MOL001925 | Paeoniflorin_qt | 68.18 | 0.40 | Paeoniae radix rubra |
MOL007016 | Paeoniflorigenone | 65.33 | 0.37 | Paeoniae radix rubra |
MOL006996 | 1-o-beta-d-Glucopyranosylpaeonisuffrone_qt | 65.08 | 0.35 | Paeoniae radix rubra |
MOL007022 | EvofolinB | 64.74 | 0.22 | Paeoniae radix rubra |
MOL007018 | 9-Ethyl-neo-paeoniaflorin A_qt | 64.42 | 0.30 | Paeoniae radix rubra |
MOL006992 | (2R,3R)-4-Methoxyl-distylin | 59.98 | 0.30 | Paeoniae radix rubra |
MOL007008 | 4-Ethyl-paeoniflorin_qt | 56.87 | 0.44 | Paeoniae radix rubra |
MOL007012 | 4-o-Methyl-paeoniflorin_qt | 56.7 | 0.43 | Paeoniae radix rubra |
MOL000492 | (+)-Catechin | 54.83 | 0.24 | Paeoniae radix rubra |
MOL000433 | FA | 68.96 | 0.71 | Chuanxiong rhizome |
MOL002140 | Perlolyrine | 65.95 | 0.27 | Chuanxiong rhizome |
MOL002151 | Senkyunone | 47.66 | 0.24 | Chuanxiong rhizome |
MOL002157 | Wallichilide | 42.31 | 0.71 | Chuanxiong rhizome |
MOL001494 | Mandenol | 42.00 | 0.19 | Chuanxiong rhizome |
MOL002135 | Myricanone | 40.60 | 0.51 | Chuanxiong rhizome |
MOL000359 | Sitosterol | 36.91 | 0.75 | Chuanxiong rhizome, Zingiberis rhizoma |
MOL002514 | Sexangularetin | 62.86 | 0.30 | Zingiberis rhizoma |
MOL002501 | [(1S)-3-[(E)-but-2-enyl]-2-methyl-4-oxo-1-cyclopent-2-enyl] (1R,3R)-3-[(E)-3-methoxy-2-methyl-3-oxoprop-1-enyl]-2,2-dimethylcyclopropane-1-carboxylate | 62.52 | 0.31 | Zingiberis rhizoma |
MOL002464 | 1-Monolinolein | 37.18 | 0.30 | Zingiberis rhizoma |
MOL000358 | beta-Sitosterol | 36.91 | 0.75 | Zingiberis rhizoma, Angelica sinensis radix, Foeniculi fructus, Typhae pollen |
MOL000979 | 2-Methoxyfuranoguaia-9-ene-8-one | 66.18 | 0.18 | Myrrha |
MOL001033 | Diayangambin | 63.84 | 0.81 | Myrrha |
MOL001031 | Epimansumbinol | 61.81 | 0.40 | Myrrha |
MOL001131 | Phellamurin_qt | 56.6 | 0.39 | Myrrha |
MOL001009 | Guggulsterol-VI | 54.72 | 0.43 | Myrrha |
MOL000988 | 4,17(20)-(cis)-Pregnadiene-3,16-dione | 51.42 | 0.48 | Myrrha |
MOL001013 | Mansumbinoic acid | 48.10 | 0.32 | Myrrha |
MOL000098 | Quercetin | 46.43 | 0.28 | Myrrha, Typhae pollen |
MOL001028 | (8R)-3-oxo-8-Hydroxy-polypoda -13E,17E,21-triene | 44.83 | 0.59 | Myrrha |
MOL001088 | 1α-Acetoxy-9,19-cyclolanost-24-en-3β-ol | 44.40 | 0.78 | Myrrha |
MOL006115 | Kaempferol-3-O-α-L-rhamnosyl(1→2)-β-D-glucoside_qt | 62.87 | 0.24 | Typhae pollen |
MOL000354 | Isorhamnetin | 49.60 | 0.31 | Typhae pollen |
MOL001439 | Arachidonic acid | 45.57 | 0.20 | Typhae pollen |
MOL001040 | (2R)-5,7-Dihydroxy-2-(4-hydroxyphenyl)chroman-4-one | 42.36 | 0.21 | Typhae pollen |
MOL000422 | Kaempferol | 41.88 | 0.24 | Typhae pollen |
MOL006111 | Testosterone palmitate | 34.14 | 0.71 | Typhae pollen |
MOL000449 | Stigmasterol | 43.83 | 0.76 | Angelica sinensis radix, Foeniculi fructus |
MOL000254 | Eugenol | 56.24 | 0.04 | Cinnamomi cortex |
MOL000118 | L-alpha-terpineol | 48.80 | 0.03 | Cinnamomi cortex |
MOL000198 | (R)-Linalool | 39.80 | 0.02 | Cinnamomi cortex |
MOL000475 | Anethole | 32.49 | 0.03 | Cinnamomi cortex |
MOL000991 | Cinnamaldehyde | 31.99 | 0.02 | Cinnamomi cortex |
MOL002380 | cis-Anethol | 30.86 | 0.03 | Cinnamomi cortex |
MOL000704 | Styrene | 29.55 | 0.01 | Cinnamomi cortex |
MOL000431 | Coumarin | 29.17 | 0.04 | Cinnamomi cortex |
MOL000250 | cis-Cinnamaldehyde | 27.21 | 0.02 | Cinnamomi cortex |
MOL000024 | alpha-Humulene | 22.98 | 0.06 | Cinnamomi cortex |
MOL001941 | Ammidin | 34.55 | 0.22 | Foeniculi fructus |
MOL004193 | Clarkeanidine | 86.65 | 0.54 | Corydalis rhizoma |
MOL001460 | Cryptopin | 78.74 | 0.72 | Corydalis rhizoma |
MOL004234 | 2,3,9,10-Tetramethoxy-13-methyl-5,6-dihydroisoquinolino[2,1-b]isoquinolin-8-one | 76.77 | 0.73 | Corydalis rhizoma |
MOL004071 | Hyndarin | 73.94 | 0.64 | Corydalis rhizoma |
MOL000791 | Bicuculline | 69.67 | 0.88 | Corydalis rhizoma |
MOL004195 | CORYDALINE | 65.84 | 0.68 | Corydalis rhizoma |
MOL000785 | Palmatine | 64.60 | 0.65 | Corydalis rhizoma |
MOL004191 | Capaurine | 62.91 | 0.69 | Corydalis rhizoma |
MOL004200 | Methyl-[2-(3,4,6,7-tetramethoxy-1-phenanthryl)ethyl]amine | 61.15 | 0.44 | Corydalis rhizoma |
MOL001463 | Dihydrosanguinarine | 59.31 | 0.86 | Corydalis rhizoma |
WLZ000001 | Ponalactone A | - | - | Trogopterus dung |
WLZ000002 | Uric acid | - | - | Trogopterus dung |
WLZ000003 | Wulingzhic acid | - | - | Trogopterus dung |
WLZ000004 | Japondipsaponin E1 | - | - | Trogopterus dung |
WLZ000005 | Urea | - | - | Trogopterus dung |
WLZ000006 | Trihydroxy isosterocholenic acid | - | - | Trogopterus dung |
Compounds | Degree | Betweenness centrality | Closeness centrality | Structure |
---|---|---|---|---|
Quercetin | 351 | 0.435 155 | 0.489 569 | ![]() |
beta-Sitosterol | 144 | 0.042 876 | 0.389 812 | ![]() |
Stigmasterol | 115 | 0.034 907 | 0.388 093 | ![]() |
Kaempferol | 45 | 0.074 389 | 0.399 546 | ![]() |
Ellagic acid | 30 | 0.010 948 | 0.346 798 | ![]() |
Eugenol | 29 | 0.056 086 | 0.360 287 | ![]() |
Arachidonic acid | 23 | 0.063 158 | 0.350 947 | ![]() |
Baicalein | 23 | 0.041 211 | 0.367 816 | ![]() |
Isorhamnetin | 22 | 0.025 932 | 0.368 586 | ![]() |
Trihydroxy isosterocholenic acid | 21 | 0.074 025 | 0.374 070 | ![]() |
表2 少腹逐瘀汤有效成分节点拓扑参数
Table 2 Topological parameters of the nodes of active components in Shaofu Zhuyu Decoction
Compounds | Degree | Betweenness centrality | Closeness centrality | Structure |
---|---|---|---|---|
Quercetin | 351 | 0.435 155 | 0.489 569 | ![]() |
beta-Sitosterol | 144 | 0.042 876 | 0.389 812 | ![]() |
Stigmasterol | 115 | 0.034 907 | 0.388 093 | ![]() |
Kaempferol | 45 | 0.074 389 | 0.399 546 | ![]() |
Ellagic acid | 30 | 0.010 948 | 0.346 798 | ![]() |
Eugenol | 29 | 0.056 086 | 0.360 287 | ![]() |
Arachidonic acid | 23 | 0.063 158 | 0.350 947 | ![]() |
Baicalein | 23 | 0.041 211 | 0.367 816 | ![]() |
Isorhamnetin | 22 | 0.025 932 | 0.368 586 | ![]() |
Trihydroxy isosterocholenic acid | 21 | 0.074 025 | 0.374 070 | ![]() |
Genes | Degree | Betweenness centrality | Closeness centrality |
---|---|---|---|
PTGS2 | 91 | 0.170 709 83 | 0.495 774 65 |
PTGS1 | 76 | 0.102 475 29 | 0.466 843 50 |
NCOA2 | 58 | 0.052 615 02 | 0.399 092 97 |
RXRA | 50 | 0.033 608 03 | 0.395 505 62 |
KCNH2 | 48 | 0.024 529 62 | 0.391 111 11 |
ADRB2 | 47 | 0.021 468 11 | 0.388 520 97 |
CHRM1 | 47 | 0.011 673 96 | 0.333 333 33 |
CHRM3 | 46 | 0.007 195 67 | 0.317 689 53 |
ADRA1B | 45 | 0.009 294 5 | 0.330 827 07 |
PGR | 39 | 0.047 171 16 | 0.344 422 70 |
表3 治疗靶点节点拓扑参数
Table 3 Topological parameters of the nodes of therapeutic target
Genes | Degree | Betweenness centrality | Closeness centrality |
---|---|---|---|
PTGS2 | 91 | 0.170 709 83 | 0.495 774 65 |
PTGS1 | 76 | 0.102 475 29 | 0.466 843 50 |
NCOA2 | 58 | 0.052 615 02 | 0.399 092 97 |
RXRA | 50 | 0.033 608 03 | 0.395 505 62 |
KCNH2 | 48 | 0.024 529 62 | 0.391 111 11 |
ADRB2 | 47 | 0.021 468 11 | 0.388 520 97 |
CHRM1 | 47 | 0.011 673 96 | 0.333 333 33 |
CHRM3 | 46 | 0.007 195 67 | 0.317 689 53 |
ADRA1B | 45 | 0.009 294 5 | 0.330 827 07 |
PGR | 39 | 0.047 171 16 | 0.344 422 70 |
Gene targets | Small molecule compounds | PDB ID | Binding energy/(kJ·mol-1) |
---|---|---|---|
IL6 | quercetin | 1ALU | -24.48 |
IL6 | paeoniflorin | 1ALU | -26.48 |
TNF | quercetin | 1A8M | -20.13 |
TNF | paeoniflorin | 1A8M | -14.77 |
TNF | kaempferol | 1A8M | -21.51 |
AKT1 | quercetin | 1UNQ | -24.39 |
AKT1 | baicalein | 1UNQ | -23.93 |
AKT1 | kaempferol | 1UNQ | -24.56 |
TP53 | quercetin | 1AIE | -24.56 |
TP53 | baicalein | 1AIE | -24.85 |
IL1B | quercetin | 1L2H | -18.58 |
PTGS2 | myricanone | 5F1A | -29.29 |
PTGS2 | 24240-05-9 | 5F1A | -29.16 |
PTGS2 | fumarine | 5F1A | -30.79 |
PTGS2 | myrrhanol C | 5F1A | -28.20 |
PTGS2 | myrrhanones B | 5F1A | -24.18 |
PTGS2 | 15α-hydroxymansumbinone | 5F1A | -32.17 |
PTGS2 | 3-methoxyfuranoguaia-9- en-8-one | 5F1A | -24.31 |
ESR1 | ST057701 | 1L2I | -28.87 |
EGFR | quercetin | 4I22 | -29.62 |
表4 少腹逐瘀汤活性成分与关键靶点的分子对接 (Continued from previous page)
Table 4 Molecular docking of active components of Shaofu Zhuyu Decoction to key targets
Gene targets | Small molecule compounds | PDB ID | Binding energy/(kJ·mol-1) |
---|---|---|---|
IL6 | quercetin | 1ALU | -24.48 |
IL6 | paeoniflorin | 1ALU | -26.48 |
TNF | quercetin | 1A8M | -20.13 |
TNF | paeoniflorin | 1A8M | -14.77 |
TNF | kaempferol | 1A8M | -21.51 |
AKT1 | quercetin | 1UNQ | -24.39 |
AKT1 | baicalein | 1UNQ | -23.93 |
AKT1 | kaempferol | 1UNQ | -24.56 |
TP53 | quercetin | 1AIE | -24.56 |
TP53 | baicalein | 1AIE | -24.85 |
IL1B | quercetin | 1L2H | -18.58 |
PTGS2 | myricanone | 5F1A | -29.29 |
PTGS2 | 24240-05-9 | 5F1A | -29.16 |
PTGS2 | fumarine | 5F1A | -30.79 |
PTGS2 | myrrhanol C | 5F1A | -28.20 |
PTGS2 | myrrhanones B | 5F1A | -24.18 |
PTGS2 | 15α-hydroxymansumbinone | 5F1A | -32.17 |
PTGS2 | 3-methoxyfuranoguaia-9- en-8-one | 5F1A | -24.31 |
ESR1 | ST057701 | 1L2I | -28.87 |
EGFR | quercetin | 4I22 | -29.62 |
图8 少腹逐瘀汤关键成分与作用靶点分子对接信息A. The docking diagram of IL1B with Quercetin; B. The docking diagram of EGFR with Quercetin; C. The docking diagram of ESR1 with ST057701; D. The docking diagram of TP53 with Baicalein; E. The docking diagram of AKT1 with Kaempferol; F. The docking diagram of TNF with Kaempferol; G. The docking diagram of PTGS2 with Fumarine; H. The docking diagram of IL6 with Paeoniflorin
Fig.8 Molecular docking information of key components of Shaofu Zhuyu Decoction to targets
图9 少腹逐瘀汤对HeLa和HaCaT细胞存活率的影响A. Effect of Shaofu Zhuyu Decoction on survival rates of HeLa cells; B. Effect of Shaofu Zhuyu Decoction on survival rates of HaCaT cells
Fig.9 Effect of Shaofu Zhuyu Decoction on survival rates of HeLa and HaCaT cells
图11 细胞凋亡检测结果A. Apoptosis detected by ?ow cytometry; B. Statistical analysis of cell proportion in different stages of apoptosis
Fig.11 Results of cell apoptosis
图12 少腹逐瘀汤对HeLa细胞凋亡相关信号通路的调控A. Protein expression levels of the CON group, Shaofu Zhuyu Decoction group on the PI3K/AKT/Bcl-2 signaling pathway; B. Statistical analysis of the expression levels of related proteins
Fig.12 Shaofu Zhuyu Decoction regulates the apoptosis signaling pathway in HeLa cells
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