中华医学超声杂志

应用化学 ›› 2011, Vol. 28 ›› Issue (08): 956-962.DOI: 10.3724/SP.J.1095.2011.00598

• 研究论文 • 上一篇    下一篇

川芎水蒸气蒸馏和超临界CO2提取物化学成分的GC-MS分析鉴别

曾志1,2*,谢润乾1,谭丽贤1,张涛3   

  1. (1.华南师范大学化学与环境学院 广州  510631;
    2.Faculty of Bioresource Sciences,Akita Prefectural University,Akita 010-0195,Japan;
    3.华南师范大学光电材料与技术研究所 广州)
  • 收稿日期:2010-10-11 修回日期:2010-12-24 出版日期:2011-08-10 发布日期:2011-08-10
  • 通讯作者: 曾志，教授; Tel:020-85217283; Fax:020-39310187; E-mail:zhizeng@scnu.edu.cn; 研究方向：天然产物化学
  • 基金资助:
    广东省科技计划重大专项(A301020101)，广东省自然科学基金(01142)资助项目

GC-MS Analysis and Determination of Chemical Components of the Extracts from Ligusticum Chuanxiong Hort. by Steam Distillation and Supercritical CO2 Extraction

ZENG Zhi1,2*, XIE Runqian1, TAN Lixian1, ZHANG Tao3   

  1. (1.School of Chemistry and Environment,South China Normal University,Guangzhou 510631;
    2.Faculty of Bioresource Sciences,Akita Prefectural University,Akita 010-0195,Japan;
    3.Institute of Opto-electronic Materials and Technology,South China Normal University,Guangzhou)
  • Received:2010-10-11 Revised:2010-12-24 Published:2011-08-10 Online:2011-08-10

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摘要：

用水蒸气蒸馏和超临界CO2提取方法对川芎药材中的化学成分进行提取，用气相色谱-质谱联用技术(GC-MS)对提取物进行分析，分别鉴定出30和34个化合物，测定了其相对含量。 结果表明，水蒸气蒸馏和超临界CO2提取物的主要成分均为(Z)-藁本内酯。 超临界CO2提取方法能提取出较多的川芎药效物质，如丁基酞内酯、丁烯基酞内酯、川芎内酯A、(Z)-藁本内酯、(E)-藁本内酯和川芎内酯I。 而水蒸气蒸馏提取物中除了川芎药效物质外，还含有较多的萜烯类化合物。 结果表明，超临界CO2对川芎药效物质的提取比水蒸气蒸馏法的效率高。 通过对川芎中带不饱和侧链与饱和侧链内酯类化合物质谱裂解规律的解析归纳，鉴别了川芎中不饱和侧链内酯类化合物如(Z)-藁本内酯、(E)-藁本内酯、丁烯基酞内酯和川芎内酯I以及饱和侧链内酯类化合物如丁基酞内酯和川芎内酯A。

关键词: 川芎, 水蒸气蒸馏, 超临界CO2提取, 质谱, 化学成分, 结构鉴别

Abstract:

The chemical components of Ligusticum chuanxiong Hort. were extracted by steam distillation and supercritical CO2 extraction, and analyzed by combined capillary gas chromatography-mass spectrometry(GC-MS). Thirty and thirty four compounds were identified, respectively, and their relative contents were determined. This study revealed that the major components of L.chuanxiong Hort. by steam distillation and supercritical CO2 extraction were (Z)-ligustilide. The chemical components from L.chuanxiong Hort. extracted by supercritical CO2 extraction possessed more bioactive substances such as butylphthalide, butylidene phthalide, senkyunolide A, (Z)-ligustilide, (E)-ligustilide and senkyunolide I. However, the components from L.chuanxiong Hort. extracted by steam distillation contained more terpenes except for the bioactive substances. Therefore, supercritical CO2 extraction has more productivity than steam distillation for the extraction of the bioactive substances from L.chuanxiong Hort. Through the interpretation and summarization of the regular patterns of the lactones with unsaturated and saturated side chains from L.chuanxiong Hort., the structures of the lactones with unsaturated side chain such as (Z)-ligustilide, (E)-ligustilide, butylidene phthalide and senkyunolide I as well as the lactones with saturated side chain such as butylphthalide and senkyunolide A have been determined.

Key words: Ligusticum chuanxiong Hort., steam distillation, supercritical CO2 extraction, mass spectrometry, chemical component, structure determination

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