部分析因法分析油包水型聚乙烯亚胺水溶液/石蜡乳液的稳定性

doi:10.11944/j.issn.1000-0518.2018.10.170349

摘要/Abstract

摘要：

聚乙烯亚胺(PEI)及其乳状液被广泛用于酶固定化、基因治疗、污水处理等领域。为提高PEI乳状液的稳定性,促进其应用,本文采用部分析因试验法考察了影响PEI水溶液/石蜡乳状液(W/O)稳定性的主因素和各因素间交互作用。结果表明,乳化剂用量和PEI的水相质量分数是影响PEI水溶液/石蜡乳液稳定性的主因素。 PEI的水相质量分数和油/水体积比的交互作用最显著。优化后,制备最稳定的PEI水溶液/石蜡乳状液(W/O)的条件是:m(Span-80):m(Tween-80)=6:1,PEI的水相质量分数为5%,乳化剂用量为0.07 g/mL,V(石蜡):V(PEI水溶液)=6:4,均质时间为3 min,转速为6000 r/min。在优化条件下制备的PEI水溶液/石蜡乳状液在放置7 d后乳状液的外观无明显变化,这表明所制备的PEI水溶液/石蜡乳状液具有良好的稳定性。

关键词: 聚乙烯亚胺水溶液/石蜡乳液, 稳定性, 部分析因试验, 交互作用

Abstract:

Polyethyleneimine(PEI) and its emulsions have been widely used in enzyme immobilization, gene therapy and waste water treatment, etc. In this work, a partial factorial test was employed to study the main factors and the interaction effect between various factors influencing the stability of water-in-oil(W/O) type PEI solution-in-paraffin emulsion. The results show that the amount of emulsifier and the mass fraction of PEI in aqueous phase are the main factors that influence the stability of the emulsion. The most significant factor is the interaction between the mass fraction of PEI in aqueous phase and the volume ratio of water/oil. The optimum conditions of the emulsion are:20% PEI in the aqueous phase, 0.07 g/mL of the emulsifier, 6:4 volume ratio of water/oil, 3 min of homogenization time and 6000 r/min of the homogenization velocity. The emulsion prepared under the optimal conditions has shown no significant changes of emulsification in a week, indicating its excellent stability.

Key words: polyethyleneimine solution-in-paraffin emulsion, stability, partial factors test, factors interaction

黄登发,刘严华,王峰. 部分析因法分析油包水型聚乙烯亚胺水溶液/石蜡乳液的稳定性[J]. 应用化学, 2018, 35(10): 1234-1242.

HUANG Dengfa,LIU Yanhua,WANG Feng. Partial Factorial Analysis for the Stability of Water-in-Oil Type Polyethyleneimine Solution-in-Paraffin Emulsion[J]. Chinese Journal of Applied Chemistry, 2018, 35(10): 1234-1242.

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