Substrate Design of Surface-Enhanced Raman Spectroscopy and Research Progress in the Detection of Body Fluids

doi:10.19894/j.issn.1000-0518.240199

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (1): 1-13.DOI: 10.19894/j.issn.1000-0518.240199

• Review •

Substrate Design of Surface-Enhanced Raman Spectroscopy and Research Progress in the Detection of Body Fluids

Yun-Fan CAI¹^,², Ke-Hui XU², Yao ZHAO², Zhi-Ting LI¹^,², Ling-Yun XIA¹, Chen-Yu WANG², Zhi-Xiao LUO¹(), Li-Na NIU²()

^1.Department of Stomatology，Taihe Hospital，Hubei University of Medicine，Shiyan 442000，China
^2.Department of Prosthodontics，School of Stomatology，Air Force Medical University，Shaanxi Key Laboratory of Stomatology，National Clinical Research Center for Oral Diseases，State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration，Xi'an 710032，China

Received:2024-07-01 Accepted:2024-11-20 Published:2025-01-01 Online:2025-01-24
Contact: Zhi-Xiao LUO,Li-Na NIU
About author:niulina831013@126.com；
13872756277@163.com
Supported by:
the Shaanxi Key Scientific and Technological Innovation Team(2020TD-033);the National Clinical Research Center for Oral Diseases(LCB202209)

Abstract

Abstract:

Surface enhanced Raman spectroscopy （SERS） has been extensively studied in the field of medical testing due to its unique advantages of rapid detection， high sensitivity， low detection limits and non-destructive analysis. The successful construction of SERS substrates is essential for realizing these benefits. In this paper， the enhancement mechanism of SERS is introduced， the design of its enhanced substrate in blood detection is categorized and discussed. It is divided into two categories： label-free substrates and label substrates， and further subdivided according to different material applications and substrate design methods. A review of relevant literature on the application of SERS detection in blood testing in recent years， summarizes the design methods and application characteristics of different types of enhanced substrates in blood testing. Finally， the design of SERS-enhanced substrate in blood examination is summarized and prospected.

Key words: Surface-enhanced Raman spectroscopy, Enhancement mechanism, Label-free substrate, Label substrate, Blood testing

CLC Number:

O657.3

Yun-Fan CAI, Ke-Hui XU, Yao ZHAO, Zhi-Ting LI, Ling-Yun XIA, Chen-Yu WANG, Zhi-Xiao LUO, Li-Na NIU. Substrate Design of Surface-Enhanced Raman Spectroscopy and Research Progress in the Detection of Body Fluids[J]. Chinese Journal of Applied Chemistry, 2025, 42(1): 1-13.

Figures/Tables 7

Fig.1 Research on the application of SERS-enhanced substrates in body fluid testing

Fig.2 Schematic diagram of a gold nanosheet made from a template with rose petals［21］

Fig.3 TEM image of Au Sts （A） and size distribution obtained statistically from TEM images （B） of Au Sts［51］

Fig.4 （A） Schematic diagram of three-dimensional Ni-NiO nano-structures synthesized on Ni substrate by femtosecond laser ablation physical synthesis technology；（B） TEM image of a synthesized Ni-NiO nanostructure［58］

Fig.5 Schematic diagram and detection process of Au-coated TiO2 MIO reinforced substrate［72］

Fig.6 （A） Schematic diagram of VP40 antibody encapsulated in gold-silica （SiO2） core-Shell material；（B） Schematic of Ebola， Lassa， and malaria detection using SERS nanotag technology［74］

Fig.7 （A） Schematic diagram of Au NR and Au@4-MBN@Ag NP synthesized and functionalized with DNA probes that are semi-complementary to the miRNA-21 sequence， respectively；（B） Characteristic peaks in the Au@4-MBN@Ag NP reporter Raman signal silencing window （1800~2800 cm-1）［77］

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Substrate Design of Surface-Enhanced Raman Spectroscopy and Research Progress in the Detection of Body Fluids

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