Ratio Fluorescence Sensor Based on Carbon Nitride Quantum Dots/Rhodamine B System for Mercury Ion Detection

doi:10.19894/j.issn.1000-0518.230166

Chinese Journal of Applied Chemistry ›› 2023, Vol. 40 ›› Issue (11): 1550-1557.DOI: 10.19894/j.issn.1000-0518.230166

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Ratio Fluorescence Sensor Based on Carbon Nitride Quantum Dots/Rhodamine B System for Mercury Ion Detection

Ye-Hong ZHOU¹, Xu-Yi ZHANG¹, Dong-Tao LU¹, Hui-Wen XU², Yang LIU¹(), Chuan DONG¹()

^1.Institute for Environmental Science，Shanxi University，Taiyuan 030006，China
^2.Taiyuan Heavy Industry Rail Transit Equipment Company Limited，Taiyuan 030032，China

Received:2023-06-05 Accepted:2023-10-05 Published:2023-11-01 Online:2023-12-01
Contact: Yang LIU,Chuan DONG
About author:DC@sxu.edu.cn
4747@sxu.edu.cn
Supported by:
the National Natural Science Foundation of China(22274090)

Abstract

Abstract:

Blue carbon nitride quantum dots （CNQDs） have been prepared from citric acid and urea by one-step hydrothermal method， characterized for morphology， elemental composition， surface functional groups， and particle size distribution. The photophysics of CNQDs is also investigated for the fluorescent sensing of Hg²⁺ by coupling with rhodamine B （RhB）. CNQDs/RhB could sense Hg²⁺ with high sensitivity and selectivity from the intensity ratio of F₄₄₇/F₅₈₁. The sensing linear range is from 2.0 to 40 μmol/L， with the detection limit as 1.95 μmol/L. The common anions and cations do not interfere the detection of Hg²⁺by CNQDs/RhB. The sensor has been applied for the detection of Hg^{2 +} in real water samples， suggesting a great application potential.

Key words: Fluorescent sensor, Carbon dots, Hg²⁺, Environmental monitoring

CLC Number:

O657.3

Ye-Hong ZHOU, Xu-Yi ZHANG, Dong-Tao LU, Hui-Wen XU, Yang LIU, Chuan DONG. Ratio Fluorescence Sensor Based on Carbon Nitride Quantum Dots/Rhodamine B System for Mercury Ion Detection[J]. Chinese Journal of Applied Chemistry, 2023, 40(11): 1550-1557.

Figures/Tables 14

Fig.1 （A） TEM image of CNQDs， inset is the HRTEM；（B） XRD spectrum of CNQDs；（C） XPS spectrum of CNQDs；（D） FT-IR spectrum of CNQDs；（E） The absorption， excitation and emission spectra of CNQDs；（F） The excitation dependent fluorescence spectra of CNQDs

Fig.2 （A） The fluorescence spectra of CNQDs/RhB；（B） The effect of pH on the fluorescence intensity of Hg2+ sensed by CNQDs/RhB systems

Fig.3 The effect of reaction time on the fluorescence intensity of Hg2+ detected by CNQDs/RhB systems

Fig.4 The effect of CNQDs mass concentration on the fluorescence intensity of Hg2+ detection

Fig.6 The concentration effect of Hg2+ on the fluorescent intensity

Fig.7 The plot of fluorescent intensity ration against the concentration

Fig.8 The selectivity of CNQDs/RhB towards different metal ions

Fig.9 The selectivity of CNQDs/RhB towards different anions

Fig.10 The interference of different metal ions for the detection of Hg2+

Fig.11 The interference of different anions for the detection of Hg2+

Fig.12 The emission spectra of CNQDs/RhB sensing system at different temperature

Fig.13 The lifetime of sensing system at different temperature

Table 1 The detection ability of CNQDs/RhB ratio fluorescent probes for Hg2+ in water samples

Water samples	Added/（μmol·L^-1）	Found/（μmol·L^-1）	Recovery/%	RSD/%（n=3）
Tap water	5.00	5.29	105.8	4.8
	10.00	10.12	101.2	3.3
	35.00	35.91	102.6	1.5
Lake	5.00	5.91	110.2	6.8
	10.00	11.00	110	4.8
	35.00	34.58	98.8	1.4

References 29

1	POMAL N C， BHATT K D， MODI K M， et al. Functionalized silver nanoparticles as colorimetric and fluorimetric sensor for environmentally toxic mercury ions： an overview［J］. J Fluorescence， 2021， 31（3）： 635-649.
2	RÍOS M C， BRAVO N F， SÁNCHEZ C C， et al. Chemosensors based on N-heterocyclic dyes： advances in sensing highly toxic ions such as CN- and Hg²⁺［J］. RSC Adv， 2021， 11（54）： 34206-34234.
3	ZHANG L， WANG Y， HUANG J， et al. Azido chelating fiber： synthesis， characterization and adsorption performances towards Hg²⁺ and Pb²⁺ from water［J］. Polym Adv Technol， 2017， 28（11）： 1418-1427.
4	FITRI Z， ADLIM M， SURBAKTI M S， et al. Mercury（II） ions assessment as a toxic waste hazard in solution based on imagery data for a part of environmental disaster management； proceedings of the IOP Conference Series： Earth and Environmental Science， F， 2019［C］. IOP Publishing.
5	HASSAN A M， AHMED H M， ABOUL-ENEIN H Y. New simple ion-selective membrane electrode for serious environmental pollutant， mercury（II）， analysis in aqueous solution， fluorescent mercury lamp white dust， mercurochrome and dental alloy［J］. Curr Anal Chem， 2018， 14（1）： 36-42.
6	SONG S， LI Y， LIU Q S， et al. Interaction of mercury ion （Hg²⁺） with blood and cytotoxicity attenuation by serum albumin binding［J］. J Hazard Mater， 2021， 412： 125158.
7	HE L， LU Y， WANG F， et al. Bare eye detection of Hg（II） ions based on enzyme inhibition and using mercaptoethanol as a reagent to improve selectivity［J］. Microchim Acta， 2018， 185（3）： 1-8.
8	SERAFIMOVSKI I， KARADJOVA I， STAFILOV T， et al. Determination of inorganic and methylmercury in fish by cold vapor atomic absorption spectrometry and inductively coupled plasma atomic emission spectrometry［J］. Microchem J， 2008， 89（1）： 42-47.
9	LIU P， PTACEK C J， BLOWES D W， et al. Mechanisms of mercury removal by biochars produced from different feedstocks determined using X-ray absorption spectroscopy［J］. J Hazard Mater， 2016， 308： 233-242.
10	DOMANICO F， FORTE G， MAJORANI C， et al. Determination of mercury in hair： comparison between gold amalgamation-atomic absorption spectrometry and mass spectrometry［J］. J Trace Elements Med Biol， 2017， 43： 3-8.
11	THIRUMALAI M， KUMAR S N， PRABHAKARAN D， et al. Dynamically modified C18 silica monolithic column for the rapid determinations of lead， cadmium and mercury ions by reversed-phase high-performance liquid chromatography［J］. J Chromatogr A， 2018， 1569： 62-69.
12	LIMA A F， DA COSTA M C， FERREIRA D C， et al. Fast ultrasound-assisted treatment of inorganic fertilizers for mercury determination by atomic absorption spectrometry and microwave-induced plasma spectrometry with the aid of the cold-vapor technique［J］. Microchem J， 2015， 118： 40-44.
13	KUMAR A， AHMED N. Indirect approach for CN-detection： development of “naked-eye” Hg²⁺-induced turn-off fluorescence and turn-on cyanide sensing by the Hg²⁺ displacement approach［J］. Ind Eng Chem Res， 2017， 56（22）： 6358-6368.
14	CHEN J， GAO Y， HU X， et al. Detection of hydroquinone with a novel fluorescence probe based on the enzymatic reaction of graphite phase carbon nitride quantum dots［J］. Talanta， 2019， 194： 493-500.
15	ZHANG Q， LIU Y， NIE Y， et al. Wavelength-dependent surface plasmon coupling electrochemiluminescence biosensor based on sulfur-doped carbon nitride quantum dots for K-RAS gene detection［J］. Anal Chem， 2019， 91（21）： 13780.
16	DANG X， ZHAO H， WANG X， et al. Photoelectrochemical aptasensor for sulfadimethoxine using g-C₃N₄ quantum dots modified with reduced graphene oxide［J］. Microchim Acta， 2018， 185（7）： 1-8.
17	GU S， HSIEH C T， GANDOMI Y A， et al. Microwave growth and tunable photoluminescence of nitrogen-doped graphene and carbon nitride quantum dots［J］. J Mate Chem C， 2019， 7（18）： 5468-5476.
18	LI L， WANG J， XU S， et al. Recent progress in fluorescent probes for metal ion detection［J］. Frontiers Chem， 2022， 10： 875241-875256.
19	JAKIMIŃSKA A， PAWLICKI M， MACYK W. Photocatalytic transformation of rhodamine B to rhodamine-110-the mechanism revisited［J］. J Photochem Photobiol A， 2022， 433： 114176-83.
20	CHENG X， LI S， GONG M， et al. Novel ratiometric fluorescent probe based on internal reference and its detection of hydrazine［J］. J Fluoresc， 2022， 32（3）： 1135-1141.
21	HAMD-GHADAREH S， HAMAH-AMEEN B A， SALIMI A， et al. Ratiometric enhanced fluorometric determination and imaging of intracellular microRNA-155 by using carbon dots， gold nanoparticles and rhodamine B for signal amplification［J］. Microchim Acta， 2019， 186（7）： 1-12.
22	TAO H， ZHANG Z， CAO Q， et al. Ratiometric fluorescent sensors for nitrite detection in the environment based on carbon dot/rhodamine B systems［J］. RSC Adv， 2022， 12（20）： 12655-12662.
23	YANG Y， XIAO X， XING X， et al. Rhodamine B assisted graphene quantum dots flourescent sensor system for sensitive recognition of mercury ions［J］. J Lumin， 2019， 207： 273-281.
24	LI H， ZHANG C， WANG J， et al. Pristine graphic carbon nitride quantum dots for the visualized detection of latent fingerprints［J］. Anal Sci， 2021， 37（11）： 1497-1503.
25	MOUSAVI A， ZARE-DORABEI R， MOSAVI S H. Sensitive detection of tamsulosin hydrochloride based on dual-emission ratiometric fluorescence probe consisting of amine-carbon quantum dots and rhodamine B［J］. Sci Rep， 2021， 11（1）： 20805-20815.
26	LIN L， ZOU C. Kinetic and thermodynamic study of magnetic separable β-cyclodextrin inclusion complex with organic phosphoric acid applied to removal of Hg²⁺［J］. J Chem Eng Data， 2017， 62（2）： 762-772.
27	LIN S Y， ZHU H， XU W J， et al. A squaraine based fluorescent probe for mercury ion via coordination induced deaggregation signaling［J］.Chin Chem Lett， 2014， 25（9）： 1291-1295.
28	HOLLETT G， ROBERTS D S， SEWELL M， et al. Quantum ensembles of silicon nanoparticles： discrimination of static and dynamic photoluminescence quenching processes［J］. J Phys Chem C， 2019， 123（29）： 17976-17986.
29	CIOTTA E， PROSPOSITO P， PIZZOFERRATO R. Positive curvature in Stern-Volmer plot described by a generalized model for static quenching［J］. J Lumin， 2019， 206： 518-522.

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Ratio Fluorescence Sensor Based on Carbon Nitride Quantum Dots/Rhodamine B System for Mercury Ion Detection

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