Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection

doi:10.11944/j.issn.1000-0518.2018.07.170312

Chinese Journal of Applied Chemistry ›› 2018, Vol. 35 ›› Issue (7): 842-848.DOI: 10.11944/j.issn.1000-0518.2018.07.170312

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Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection

Jianping YANG(),Tingting SU,Xiaozhen QIU,Hanjie ZHANG,Yali WANG,wei YIN

Engineering Technology Development Center of Advanced Materials & Energy Saving and Emission Reduction in Guangdong Colleges and Universities,Department of Chemistry,Guangdong University of Education,Guangzhou 510303,China

Received:2017-09-01 Accepted:2017-11-29 Published:2018-07-06 Online:2018-07-06
Contact: Jianping YANG
Supported by:
Supported by the Natural Science Foundation of Guangdong Province(No.2015A030310202), Higher Education Reformation Projects(No.2016ybzz08), Innovative Training Program For College Students(No.201714278005, No.201714278075), Project of Engineering Technology Development Center of Advanced Materials & Energy Saving and Emission Reduction in Guangdong Colleges and Universities(No.2016GCZX007)

Abstract

Abstract:

A novel method for the extraction, isolation, identification and determination of the active component of Chinese medicine and its inhibitory activity on glucoamylase in a simple and efficient way was developed. The active ingredient baicalin was extracted from Scutellaria baicalensis using green nontoxic water-extracting and acid-depositing method. It was identified qualitatively by thin layer chromatography and the content was determined by ultraviolet spectrophotometry. An effective electrochemical glucose sensor was fabricated and a new method of characterizing hypoglycemic activity was developed based on electrochemical amperometric detection. Acarbose(clinical antidiabetic drug) was further used to demonstrate the reliability and stability of determining the inhibitory activity of glucoamylase by electrochemical amperometry. The results show that the baicalin has a good inhibitory activity on glucoamylase with a half maximal inhibitory concentration(IC₅₀) at 0.056 g/L. This study provides a novel method for the extraction and isolation of hypoglycemic components from natural medicines.

Key words: baicalin, glucoamylase, electrochemical method, inhibitory activity

Jianping YANG, Tingting SU, Xiaozhen QIU, Hanjie ZHANG, Yali WANG, wei YIN. Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection[J]. Chinese Journal of Applied Chemistry, 2018, 35(7): 842-848.

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Screening of Hypoglycemic Constituents in Chinese Herbal Medicine Based on Electrochemical Amperometric Detection

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