Preparation and Enzymatic Characterizations of Hydrophobic Poly(acrylic acid)/Polyvinyl Alcohol/Glucoamylase Composite Nanofiber Membrane

doi:10.11944/j.issn.1000-0518.2015.12.150143

Chinese Journal of Applied Chemistry ›› 2015, Vol. 32 ›› Issue (12): 1364-1370.DOI: 10.11944/j.issn.1000-0518.2015.12.150143

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Preparation and Enzymatic Characterizations of Hydrophobic Poly(acrylic acid)/Polyvinyl Alcohol/Glucoamylase Composite Nanofiber Membrane

SUI Chunhong(),WANG Cheng,WEI yuqing,ZHAI Huan,LI Nan,DONG Shunfu,HAN Liqin

Jilin Medical University,Jilin,Jilin 132013,China

Received:2015-04-15 Accepted:2015-09-25 Published:2015-12-01 Online:2015-12-01
Contact: SUI Chunhong
Supported by:
Supported by the Education Department Foundation of Jilin Province of China(No.2013354), Jilin Province Science & Technology Department Foundation of China(No.20150101131JC)

Abstract

Abstract:

Glucoamylase(GA) was entrapped and immobilized in the poly(acrylic acid)(PAA)/polyvinyl alcohol(PVA) nanofibers membrane by the mixed liquid electrospinning entrapping method. The enzyme immobilization potential of PAA/PVA was investigated through appraised enzymatic characterization of immobilized GA. Its physicochemical properties and enzymatic characterizations were identified. Fourier transform infrared spectrum(FT-IR) and scanning electron microscope(SEM) photographs indicate that GA can be successfully entrapped in the interior of the electrospinning PAA/PVA composite nanofiber membrane. The optimum reaction temperature of immobilized GA is 68 ℃, 9 degrees higher than that of free GA, and the applicable pH range of immobilized GA is wider than that of free GA significantly. In addition, immobilized GA also has good thermal stability, storage stability and reusability. PAA/PVA nanofiber membrane is a good carrier for enzyme immobilization with potential application. The protein molecules can be easily fixed into the inside of nanofiber membrane via mixed liquid electrospinning entrapping method.

Key words: electrospinning, nanofiber membrane, glucoamylase, entrapping method, immobilization

SUI Chunhong, WANG Cheng, WEI yuqing, ZHAI Huan, LI Nan, DONG Shunfu, HAN Liqin. Preparation and Enzymatic Characterizations of Hydrophobic Poly(acrylic acid)/Polyvinyl Alcohol/Glucoamylase Composite Nanofiber Membrane[J]. Chinese Journal of Applied Chemistry, 2015, 32(12): 1364-1370.

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Preparation and Enzymatic Characterizations of Hydrophobic Poly(acrylic acid)/Polyvinyl Alcohol/Glucoamylase Composite Nanofiber Membrane

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