Rapid Analysis of Chemical Composition in Asparagus Using Internal Extractive Electrospray Ionization Mass Spectrometry

doi:10.11944/j.issn.1000-0518.2016.04.150315

Chinese Journal of Applied Chemistry ›› 2016, Vol. 33 ›› Issue (4): 481-488.DOI: 10.11944/j.issn.1000-0518.2016.04.150315

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Rapid Analysis of Chemical Composition in Asparagus Using Internal Extractive Electrospray Ionization Mass Spectrometry

LU Haiyan^a,ZHANG Hua^a,PENG Xiaoming^b,CHEN Huanwen^a^*()

^aJiangxi Key Laboratory for Mass Spectrometry and Instrumentation,East China Institute of Techonolgy,Nanchang 330013,China
^bXingguo Xian Boyuan Agricultural Development Co. Ltd.,Xingguo,Jiangxi 342400,China

Received:2015-08-21 Accepted:2015-11-20 Published:2016-04-01 Online:2016-04-01
Contact: CHEN Huanwen
Supported by:
Supported by the National Natural Science Foundation of China(No.21565004), Program for Changjiang Scholars and Innovative Research Team in University(PCSIRT)(No.IRT13054), Science and Technology Planning Project at the Department of Science and Technology of Jiangxi Province, China(No.20142BCB24005), Jiangxi Province Program for the support of Science and Technology in Universities(No.KJLD13051)

Abstract

Abstract:

Rapid analysis chemical composition of plant samples was achieved using internal extractive electrospray ionization mass spectrometry(iEESI-MS) without any sample pretreatment. In this study, fresh asparagus tubers together with two kinds of dry asparagus tubers were treated as representative samples for the iEESI-MS analysis. Choosing methanol as an iEESI extractant, various chemical composition in asparagus tissue samples was profiled in mass scan range of m/z 50~1000 under the positive ion detection mode, and through collision-induced dissociation(CID) experiments, nutrient and medicinal components including sugars, amino acids, alkaloids, and rutin were identified, which play an significant role in development of pharmaceutical drugs, natural product research and understanding of primary and secondary metabolites. Our experimental results demonstrate that iEESI-MS makes rapid recognition of chemical composition differences in different asparagus. Such information renders the nutritious and medicinal assessment of plant natural products possible. The merits of iEESI-MS include no sample pretreatment, high-throughput(less than 1 min per sample), low sample consumption, simplicity, as well as good specificity, showing potential applications in phytochemicals analysis.

Key words: asparagus, internal extractive electrospray ionization, mass spectrometry, direct analysis

LU Haiyan, ZHANG Hua, PENG Xiaoming, CHEN Huanwen. Rapid Analysis of Chemical Composition in Asparagus Using Internal Extractive Electrospray Ionization Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, 2016, 33(4): 481-488.

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Rapid Analysis of Chemical Composition in Asparagus Using Internal Extractive Electrospray Ionization Mass Spectrometry

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