Characterization of Components in Huangying Kechuan Syrup by Ultra-high Liquid Chromatography Tandem  Quadrupole-time-of-flight Mass Spectrometry

doi:10.19894/j.issn.1000-0518.200305

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (3): 276-288.DOI: 10.19894/j.issn.1000-0518.200305

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Characterization of Components in Huangying Kechuan Syrup by Ultra-high Liquid Chromatography Tandem Quadrupole-time-of-flight Mass Spectrometry

YAN Yi-Meng^1,2, YUE Ke-Xin^1,2, LIU Yu-Sheng³, CHEN Ge⁴, TIAN Han-Wen⁴, LIU Zhong-Ying², LIU Zhi-Qiang¹, SONG Feng-Rui¹, PI Zi-Feng^1*

¹Center of Mass Spectrometry in Changchun, Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
²College of Pharmacy, Jilin University, Changchun 130021, China
³Jilin Academy of Chinese Medicine Sciences, Changchun 130021, China
⁴Changchun People Pharmaceutical Group Co. LTD, Changchun 130033, China

Received:2020-10-10 Accepted:2020-12-31 Published:2021-03-01 Online:2021-05-01
Supported by:
Science and Technology Development Program of Jilin Province(Nos.20200708026YY, 20190902020TC)

Abstract

Abstract: The ultra-high liquid chromatography tandem quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS^E) technology was applied to analyze the chemical constituents of Huangying Kechuan Syrup (HYKCS), and UNIFI software was used to assist the analysis. A Waters CORTECS Shield RP18 C18 column (4.6 mm×100 mm, 2.7 μm) was used with methanol (A)-0.1% formic acid solution (B) as the mobile phase for gradient elution Electrospray (ESI) ion source was used for mass spectrometry, and UPLC-Q-TOF-MS^E technology was used to collect mass spectrometry data in positive and negative ion modes. The precise molecular weights and tandem mass spectrometry data of the compound were analyzed with the UNIFI software according to the spectrum information and related references. Finally, a total of 93 compounds were inferred and identified, including 9 alkaloids, 43 flavonoids, 21 triterpenes and triterpene saponins, 2 cyanide glycosides,13 organic acids and 6 other compounds. UPLC-Q-TOF-MS^E technology could be used to analyze the total components of Huangying Kechuan Syrup rapidly and efficiently. This study provides a scientific basis for further screening the active ingredients and improving the quality control standard of the product.

Key words: Huangying Kechuan Syrup, Ultra-high liquid chromatography tandem quadrupole-time-of-flight mass spectrometry, Chemical constituents, UNIFI

CLC Number:

O657.6

YAN Yi-Meng, YUE Ke-Xin, LIU Yu-Sheng, CHEN Ge, TIAN Han-Wen, LIU Zhong-Ying, LIU Zhi-Qiang, SONG Feng-Rui, PI Zi-Feng. Characterization of Components in Huangying Kechuan Syrup by Ultra-high Liquid Chromatography Tandem Quadrupole-time-of-flight Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, 2021, 38(3): 276-288.

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Characterization of Components in Huangying Kechuan Syrup by Ultra-high Liquid Chromatography Tandem Quadrupole-time-of-flight Mass Spectrometry

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