Screening for Urine Metabolic Biomarkers of Transient Ischemic Attack

doi:10.19894/j.issn.1000-0518.210034

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (3): 305-314.DOI: 10.19894/j.issn.1000-0518.210034

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Screening for Urine Metabolic Biomarkers of Transient Ischemic Attack

ZHANG Nan¹, LI Tie², YANG Guang³, HUANG Xin¹, YUE Hao¹, WANG Yang¹, LIU Jun-Tong⁴, LIU Shu-Ying^1*, WANG Fu-Chun^2*

¹Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
²Acupuncture Massage School, Changchun University of Chinese Medicine, Changchun 130117, China
³School of Life and Environmental Sciences, Charles Perkins Centre, The University of Sydney, Sydney NSW 2006, Australia
⁴School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, China

Received:2021-01-21 Accepted:2021-02-03 Published:2021-03-01 Online:2021-05-01
Supported by:
“Thirteenth Five-Year” Science and Technology Project of Jilin Provincial Education Department (No.JJKH20200909KJ), the TCM Science and Technology Project of Jilin Province in 2019 (No.2019021), Jilin Province College Students Innovation and Entrepreneurship Training Program (No.202010199041)

Abstract

Abstract: In order to screen for the early biomarkers of transient ischemic attack (TIA) in vivo, this study was conducted to explore the metabolomic characteristics of urine in rat models of TIA caused by bilateral common carotid artery occlusion. Seven important urine metabolic markers of TIA injury, including L-malic acid, D-glucurono-6,3-lactone, L-threonine, L-valine, L-gulonolactone, ascorbate, and phosphoenolpyruvic acid, were found and identified, which were mainly involved in ascorbic acid metabolism, pyruvate metabolism and branched chain amino acid metabolism, and involved in regulating oxidative stress, inflammatory response and neuroprotection. In this study, rapid resolution liquid chromatography-mass spectrometry was used to screen in metabolic biomarkers of TIA, providing a scientific basis for the early diagnosis and treatment of stroke.

Key words: Metabonomics, Rapid resolution liquid chromatography coupled with quadruple-time-of-flight mass spectrometry, Transient ischemic attack, Metabolic biomarkers

CLC Number:

O657.6

ZHANG Nan, LI Tie, YANG Guang, HUANG Xin, YUE Hao, WANG Yang, LIU Jun-Tong, LIU Shu-Ying, WANG Fu-Chun. Screening for Urine Metabolic Biomarkers of Transient Ischemic Attack[J]. Chinese Journal of Applied Chemistry, 2021, 38(3): 305-314.

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Screening for Urine Metabolic Biomarkers of Transient Ischemic Attack

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