A Differential Study on in vitro Antioxidant Activity and Extract Composition of Different Parts of Panax Ginseng

doi:10.19894/j.issn.1000-0518.210317

Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (11): 1531-1540.DOI: 10.19894/j.issn.1000-0518.210317

A Differential Study on in vitro Antioxidant Activity and Extract Composition of Different Parts of Panax Ginseng

ZHANG Hui-E¹, HOU Jian-Feng², WANG Jing-Yuan³, ZHU Shuang¹, DU Lian-Yun¹, YE Ping¹, WEI Kun², CHEN Chang-Bao¹, LI Guang^1*, WANG En-Peng^1*

¹Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China;
²Shiqi Biological R&D Centre (Suzhou Industrial Park) Co., LTD, Suzhou 215125, China;
³The 964th Hospital of PLA, Changchun 130000, China

Received:2021-06-28 Accepted:2021-10-09 Published:2021-11-01 Online:2022-01-01
Supported by:
National Key Research and Development Projects (No.2019YFC1710704), Jilin Province Science and Technology Development Project (Nos.20200404042YY, 202005043YY, 20190201297JC, 20190304099YY) and Jiayi Biochemical Research and Development Center (Suzhou Industrial Park) Co., Ltd (No.20191120)

Abstract

Abstract: In order to provide theoretical basis for rational exploitation and utilization of ginseng resources, the differences of active antioxidants and chemical components in extracts from different parts of ginseng were compared. 1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) radical scavenging assay, 2,2'-biazo-bis-3-ethylbenzothiazoline-6-sulfone (ABTS) radical scavenging assay and ferric ion reduction assay (FRAP) were used to evaluate the antioxidant activities of different ginseng extracts. The compounds in ethyl acetate (EAE) extracts from different parts of Panax ginseng were identified and compared by gas chromatography-mass spectroscopy (GC-MS). The results of DPPH, ABTS and FRAP experiments show that the EAE extracts of ginseng stem and leaf have the best antioxidant effect. The half inhibition concentration (IC₅₀) value of DPPH is (0.66±0.02) g/L, and the IC₅₀ value of positive drug-2,6-BHT is (0.26±0.02) g/L. The IC₅₀ value of ABTS is (0.54±0.26) g/L, and the IC₅₀ value of BHT is (0.15±0.04) g/L. The EAE of FRAP test is (272.09±3.78) mg/L, and the positive BHT value is (1319.37±3.80) mg/L. By GC-MS analysis, 20, 27, 14 and 14 components are identified from the EAE extracts of ginseng root, ginseng folium, ginseng flower and ginseng seed, respectively, of which one common component is linoleic acid. This study provides a theoretical basis for better exploitation and utilization of ginseng resources and intensive processing of antioxidant food and cosmetic products.

Key words: Ginseng extract, Antioxidant activity, Chemical composition, Difference comparison

CLC Number:

O657.6
TQ658

ZHANG Hui-E, HOU Jian-Feng, WANG Jing-Yuan, ZHU Shuang, DU Lian-Yun, YE Ping, WEI Kun, CHEN Chang-Bao, LI Guang, WANG En-Peng. A Differential Study on in vitro Antioxidant Activity and Extract Composition of Different Parts of Panax Ginseng[J]. Chinese Journal of Applied Chemistry, 2021, 38(11): 1531-1540.

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A Differential Study on in vitro Antioxidant Activity and Extract Composition of Different Parts of Panax Ginseng

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