Chinese Journal of Applied Chemistry ›› 2021, Vol. 38 ›› Issue (3): 289-297.DOI: 10.19894/j.issn.1000-0518.210030

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Whitening Activity, Antioxidant Activity and Ginsenosides Analysis of Ginseng Wash Water

WANG En-Peng1, DU Lian-Yun1, JIANG Tao1, LI Guang1, WEI Kun2, ZHU Shuang1, YUE Hao1*, CHEN Chang-Bao1*   

  1. 1Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China
    2Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130117, China
  • Received:2021-01-08 Accepted:2021-02-04 Published:2021-03-01 Online:2021-05-01
  • Supported by:
    Jilin Province Science and Technology Development Project(Nos.20200404042YY, 202005043YY, 20190201297JC and 20190304099YY), the National Key Research and Development Projects (No.2019YFC1710704) and Jiayi Biochemical Research and Development Center (Suzhou Industrial Park) Co., Ltd

Abstract: The whitening, antioxidant activity and ginsenosides components of the samples of freeze-dried powder of ginseng wash water (GWW) were analyzed by measuring tyrosinase inhibition rate with biochemical enzymatic method, determining the hydroxyl radical (·OH) scavenging rate and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging rate with salicylic acid method, and high resolution rapid liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RRLC-Q-TOF MS/MS), respectively. Sixteen ginsenosides were identified. The results show that the content of total ginsenosides of the sample is 1.64 g/100 g;The sample has inhibitory effect on tyrosinase activity, and the inhibition rate is 50.50 % when the final concentration is 57.82 mg/mL. At the same time, it has certain antioxidant activity, when the sample concentration is 38.03 mg/mL, the inhibition rate of ·OH reaches 50.30%. When the sample concentration is 52.04 mg/mL, the DPPH radical scavenging rate is 50.10%. To sum up, GWW, as a waste by-product of primary processing of ginseng, has a large stock, which is worthy of recycling and re-developing as a raw material for whitening, anti-oxidation and anti-aging products.

Key words: Ginseng wash water, Whitening, Antioxidant activity, Ginsenosides, Mass spectrometry, Components analysis

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