Abstract: Hydroxypropyl-β-cyclodextrins were applied to extract and enrich isoflavones which is the functional material in Belamcanda chinensis. The extracting time and temperature were studied and the process was optimized. The amount and the category of the extrtacted isoflavones were characterized by means of UV-Vis, HPLC and HPLC-MS. Under optimized conditions, the extracting amount of isoflavones was 2 times and the extracting amount of tectoridin was 3 times as those using water as the extractant. The as-established methodology is promising to be extended to the extraction research technology of Chinese traditional drugs.

Key words: hydroxypropyl-&, beta, -cyclodextrins, Belamcanda chinensis, isoflavones, tectoridin