Abstract: The secondary metabolites of the medicinal mangrove endophytic fungus Penicillium sp.(ZZF29#) were firstly studied. Eight compounds were isolated from the ethyl acetate extract of the fermentation using silica gel column chromatography, preparative thin-layer chromatography and recrystallization. The structures of the compounds were identified as cyclo-(Phe-Ala)(1), cyclo-(Phe-Gly)(2), cyclo-(Phe-Phe)(3), cyclo-(Phe-Tyr)(4), emodin(5), physcion(6), ergosterol(7) and peroxide ergosterol(8), by comprehensive spectroscopic characterizations as well as comparison with reported data.

Key words: mangrove endophytic fungus, secondary metabolites, isolation and elucidation, cyclodipeptides, anthraquinones, sterols