Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity-Ⅱ.Synthesis and Characterization of Haptens and Antigens

Chinese Journal of Applied Chemistry ›› 1998, Vol. 0 ›› Issue (1): 53-55.

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Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity-Ⅱ.Synthesis and Characterization of Haptens and Antigens

Ma Xueyan, Wang Lin, Ding Lan, Zhao Daqing, Ni Jiazuan

Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022

Received:1997-03-25 Revised:1997-08-14 Published:1998-02-10 Online:1998-02-10

Abstract

Abstract: The thiol group of glutathione (GSH) was protected by 2, 4-dinitrochlorobenzene(DNCB).The product GSH-S-DNP was reacted with 2-methyl-1-propanol and 3-methyl-1-butanol respectively to obtain hapten6 and 7.Their structures were determined by 400 MHz ¹H NMR and IR.The two haptens were then covalently crosslinked with bovine serum albu-min(BSA) hy using glutaraldehyde as coupling agent to obtain antigen 6 and 7.The resultsof gel electrophoresis analysis gave the average molecular weight of 1.27 ?10^-22 kg for eachantigen.The average connected ratio of BSA to hapten was 1: 12.Other UV and fluores-cence spectral properties of the antigens are reported.

Key words: glutathione peroxidase, abzyme, hydrophobic hapten, preparation, hydrophobic pocket modification

Ma Xueyan, Wang Lin, Ding Lan, Zhao Daqing, Ni Jiazuan. Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity-Ⅱ.Synthesis and Characterization of Haptens and Antigens[J]. Chinese Journal of Applied Chemistry, 1998, 0(1): 53-55.

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Hydrophobic Pocket Modification and Glutathione Peroxidase Abzyme with High Activity-Ⅱ.Synthesis and Characterization of Haptens and Antigens

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