Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ. Synthesis and Property of Glutathione Derivatives as Specific Antigens

Chinese Journal of Applied Chemistry ›› 1994, Vol. 0 ›› Issue (5): 87-90.

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Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ. Synthesis and Property of Glutathione Derivatives as Specific Antigens

Zhu Zhenqi¹, Ding Lan¹, Luo Guimin¹, Sun Qi'an¹, Yang Tongshu¹, Shen Jiacong²

1. The National Laboratory of Enzyme Engineering, Jilin University, Changchun 130023;
2. Key Laboratory of Molecular Spectrum and Molecular Structure, Jilin University, Changchun

Received:1993-10-27 Revised:1994-04-05 Published:1994-10-10 Online:1994-10-10

Abstract

Abstract: The sulfhydryl groups of the reduced glutathione (GSH) have been reacted with 2,4-dinitrochlorobenzene to give S-protective glutathione as a hapten, The haptens were crosslinked with the amino groups on the surface of BSA (Bovine Serum Albumin) glutaraldehyde,The covalent conjugates are GSH specific antigens of high immunogenicity.The antigens were found to be homogenious and have a molecular weight of 87000 dalton as determined by gel-electrophoresis method.Spectral and circular dichroism measurements showed that the antigens possess special vis-UV spectra, fluorescence nature with a increased compact of antigen structure.

Key words: glutathione peroxidase, catalytic antibody, specific antigen, preparation

Zhu Zhenqi, Ding Lan, Luo Guimin, Sun Qi'an, Yang Tongshu, Shen Jiacong. Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ. Synthesis and Property of Glutathione Derivatives as Specific Antigens[J]. Chinese Journal of Applied Chemistry, 1994, 0(5): 87-90.

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Studies on Catalytic Antibody with Glutathione Peroxidase Activity Ⅰ. Synthesis and Property of Glutathione Derivatives as Specific Antigens

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