Abstract: Tiopronin(TP) capped with CdTe/CdS quantum dots(TP-CdTe/CdS QDs) was synthesized in aqueous solution. The paper utilized ultraviolet-visible(UV-Vis) absorption spectra and fluorescence spectrometry to study the interactions between TP-CdTe/CdS QDs and mitomycin C(MMC). With the optimum reaction conditions which was found to be at pH=7.6 with tris-HCl buffer solution, the quenched fluorescence intensity of TP-CdTe/CdS QDs was linearly proportional with the concentration of MMC, correlation coefficient r=0.9991, and the detection limits were in the range of 4.7×10-9~1.2×10-8 g/mL, and the detection limit(3σ) for MMC was 1.4×10-8 g/mL. The quantitative determination of MMC is quick, simple and convenient. This method has been applied to the determination of MMC in urine samples with satisfactory results.

Key words: CdTe/CdS QDs, UV-vis, Fluorescence quenching method, Mitomycin C