Abstract: Interactions of mandelic acid(MA) and bovine/goat serum albumins(BSA/GSA) have been investigated by FIA-QCM and UV-Vis spectroscopy, respectively. Based on frequency shift of quartz crystal, surface concentrations of immobilized BSA and GSA were 4.9×10-12 and 8.8×10-12 mol/cm2, respectively. After injecting mandelic acid into FIA-QCM system, frequency of the quartz crystal decreases due to interactions between enantiomers and SA molecules and the shifts between SA and R-MA are greater than those with S-enantiomer, indicating that the interactions of SA molecules with R-MA are stronger than those with S-MA. The UV binding constants for SA with S-MA are smaller than those with R-enantiomer. Taking into account the discrimination factors(α-QCM and α-UV), GSA shows a better chiral recognition with mandelic acid than BSA.

Key words: serum albumin, mandelic acid, flow injection analysis-quartz crystal microbalance, UV-Vis spectroscopy, discrimination factor