Nature of Cold and Heat and the Meaning of Compounding Within the Acrid Opening and Bitter Downbearing Groups of Banxia Xiexin Decoction Based on Energy Metabolism

doi:10.19894/j.issn.1000-0518.240416

Chinese Journal of Applied Chemistry ›› 2025, Vol. 42 ›› Issue (10): 1386-1396.DOI: 10.19894/j.issn.1000-0518.240416

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Nature of Cold and Heat and the Meaning of Compounding Within the Acrid Opening and Bitter Downbearing Groups of Banxia Xiexin Decoction Based on Energy Metabolism

Mao-Xu WANG, Yue-Gang ZHAO, Li-Ying WANG, Wei SHI, Yi-Nan LIU, Zhi-Dong QIU, Xue-Lian DONG()

College of Pharmacy，Changchun University of Chinese Medicine，Changchun 130117，China

Received:2024-12-17 Accepted:2025-05-29 Published:2025-10-01 Online:2025-10-29
Contact: Xue-Lian DONG
About author:148047753@qq.com
Supported by:
Jilin Province Science and Technology Development Plan(20220204002YY)

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Abstract

Abstract:

In order to reveal the effects of Acrid Opening and Bitter Downbearing groups of Banxia Xiexin Decoction on energy metabolism in rats before and after compatibility， and thereby exploring the changes in cold and hot properties after compounding. We established a rat model of heat syndrome through intragastric administration of Euthyrox （120 μg/kg） for a total of 15 days， monitoring alterations in physiological parameters （body mass， rectal temperature） throughout the treatment period， and assessed the hot plate reaction threshold of the rats following the final drug administration. Ultra high-performance liquid chromatography-mass spectrometry （UPLC-MS） was employed to analyze the content of energy metabolites in rat liver tissue. The results showed that compared with the model group， Huangqin （Scutellariae Radix）， Huanglian （Coptidis Rhizoma）， single decoction combination of Huangqin-Huanglian （or combined decoction）， single decoction combination of Acrid Opening and Bitter Downbearing （or combined decoction）， and four flavors single fried combination group could alleviate the signs of heat evidence， prolong the threshold of the hot plate reaction and inhibit energy metabolism in varying degrees. While the group of Banxia （Pinelliae Rhizoma）， Ganjiang （Zingiberis Rhizoma）， and single decoction combination of Banxia-Ganjiang （or combined decoction） could maintain the signs of heat evidence， reduce the threshold of the hot plate reaction and promote energy metabolism. Compared with the blank group， Huangqin， Huanglian， and single decoction combination of Huangqin-Huanglian （or combined Decoction） group decreased the rectal temperature and prolonged the threshold of hot plate reaction. While single decoction combination of Acrid Opening and Bitter Downbearing （or combined Decoction）， and four flavors single fried combination group did not have any significant changes. This indicates that Huangqin， Huanglian and the two herbs showed cold property after single decoction or combined decoction； Banxia， Ganjiang and the two herbs showed hot property after single decoction or combined decoction； and the group of Acrid Opening and Bitter Downbearing tended to be slightly cold after combined decoction， thus exerting the efficacy of “regulating the cold and heat”. In addition， the mode of decoction does not change the heat and cold properties of the drugs after combination， but it can affect the intensity of heat and cold after combination.

Key words: Banxia Xiexin Decoction, Acrid Opening and Bitter Downbearing, Herbal medicinal properties, Energy metabolism

CLC Number:

O657.6

Mao-Xu WANG, Yue-Gang ZHAO, Li-Ying WANG, Wei SHI, Yi-Nan LIU, Zhi-Dong QIU, Xue-Lian DONG. Nature of Cold and Heat and the Meaning of Compounding Within the Acrid Opening and Bitter Downbearing Groups of Banxia Xiexin Decoction Based on Energy Metabolism[J]. Chinese Journal of Applied Chemistry, 2025, 42(10): 1386-1396.

Figures/Tables 8

Table 1 Effects of medicinal flavours and their different combinations on the physical signs of rats with heat syndrome

Groups	Body mass/g	Rectal temperature/℃	Hot plate reaction threshold/t
C	310.54±5.62	37.50±0.42	17.60±1.48
M	274.22±6.62 ^b	38.40±0.36 ^b	13.67±0.38 ^b
BX	260.78±3.95	37.55±0.05	11.55±0.4 ^d
GJ	257.54±1.59	37.84±0.05	9.94±0.18 ^d
HQ	285.10±2.96 ^d	36.89±0.08 ^d	20.18±0.57 ^d
HL	288.20±3.17 ^d	36.62±0.07 ^a	21.72±0.30 ^d
CMD	314.63±5.59 ^d	37.22±0.75 ^d	29.22±1.24 ^d
HMD	278.76±15.66	38.04±0.32	10.86±0.73 ^d
CSD	303.08±12.7 ^d	36.48±0.37	28.82±0.89 ^c
HSD	281.58±12.37	37.38±0.32	12.35±0.31
HCSD	315.59±15.88 ^d	37.54±0.17	15.7±0.30 ^c
SD	318.94±13.99 ^d	37.94±0.19	21.6±1.62 ^c
MD	290.78±1.64 ^d	36.66±0.35 ^d	18.41±0.9 ^d

Fig.1 QC sample analyses：（A） QC sample negative ion mode TIC overlap plot；（B） QC sample correlation analysis

Fig.2 PCA analysis of glycolysis product content in different groupsNote： PC1 denotes the first principle component along with its contribution， whereas PC2 represents the second principal component and its contribution. The contribution denotes the capacity of the principal component to elucidate the raw data， whereas the sum of the two principal components constitutes the cumulative contribution. The dots signify the samples， the region delineated by the line linking the samples constitutes the 95% confidence interval， and the separation between the confidence intervals along with the extent of overlap indicates the variability among the distinct sample groups. The proximity between samples indicates their similarity； a shorter distance signifies greater similarity， whereas a longer distance denotes lesser similarity （Figs.4 and 6 are identical）

Fig.3 Heatmap of differential expressions of glycolytic productsNote： red represents higher levels of the relevant metabolite and blue represents lower levels of the relevant metabolite in the graph， the same below

Fig.4 PCA analysis of amino acid metabolites in different groupsNote： same comments as in Fig.2

Fig.5 Heatmap of differential expressions of amino acid metabolites

Fig.6 PCA analysis of nucleotide metabolites in different groupsNote： same comments as in Fig.2

Fig.7 Heatmap of differential expression of nucleotide metabolism

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Nature of Cold and Heat and the Meaning of Compounding Within the Acrid Opening and Bitter Downbearing Groups of Banxia Xiexin Decoction Based on Energy Metabolism

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